naa a22 4500 510739342 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12015-012-9357-8 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9357-8 Standardized Generation and Differentiation of Neural Precursor Cells from Human Pluripotent Stem Cells [Elektronische Daten] [O. Kozhich, R. Hamilton, B. Mallon] Precise, robust and scalable directed differentiation of pluripotent stem cells is an important goal with respect to disease modeling or future therapies. Using the AggreWell™400 system we have standardized the differentiation of human embryonic and induced pluripotent stem cells to a neuronal fate using defined conditions. This allows reproducibility in replicate experiments and facilitates the direct comparison of cell lines. Since the starting point for EB formation is a single cell suspension, this protocol is suitable for standard and novel methods of pluripotent stem cell culture. Moreover, an intermediate population of neural precursor cells, which are routinely >95% NCAMpos and Tra-1-60neg by FACS analysis, may be expanded and frozen prior to differentiation allowing a convenient starting point for downstream experiments. Springer Science+Business Media, LLC (outside the USA), 2012 Pluripotent stem cells nationallicence Differentiation nationallicence Neural precursor nationallicence Neurons nationallicence Kozhich O. Laboratory of Molecular Biology, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut Hamilton R. NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut Mallon B. NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 531-536 1550-8943 9:4<531 2013 9 12015 https://doi.org/10.1007/s12015-012-9357-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9357-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kozhich O. Laboratory of Molecular Biology, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Hamilton R. NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Mallon B. NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 531-536 1550-8943 9:4<531 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer