naa a22 4500 510739369 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12015-011-9336-5 doi (NATIONALLICENCE)springer-10.1007/s12015-011-9336-5 Unravelling the Pluripotency Paradox in Fetal and Placental Mesenchymal Stem Cells: Oct-4 Expression and the Case of the Emperor's New Clothes [Elektronische Daten] [Jennifer Ryan, Allison Pettit, Pascale Guillot, Jerry Chan, Nicholas Fisk] Mesenchymal stem cells (MSC) from fetal-placental tissues have translational advantages over their adult counterparts, and have variably been reported to express pluripotency markers. OCT- 4 expression in fetal-placental MSC has been documented in some studies, paradoxically without tumourogenicity in vivo. It is possible that OCT- 4 expression is insufficient to induce true "stemness”, but this issue is important for the translational safety of fetal-derived MSC. To clarify this, we undertook a systematic literature review on OCT- 4 in fetal or adnexal MSC to show that most studies report OCT- 4 message or protein expression, but no study provides definitive evidence of true OCT- 4A expression. Discrepant findings were attributable not to different culture conditions, tissue sources, or gestational ages but instead to techniques used. In assessing OCT- 4 as a pluripotency marker, we highlight the challenges in detecting the correct OCT- 4 isoform (OCT- 4A) associated with pluripotency. Although specific detection of OCT- 4A mRNA is achievable, it appears unlikely that any antibody can reliably distinguish between OCT- 4A and the pseudogene OCT- 4B. Finally, using five robust techniques we demonstrate that fetal derived-MSC do not express OCT- 4A (or by default OCT- 4B). Reports suggesting OCT- 4 expression in fetal-derived MSC warrant reassessment, paying attention to gene and protein isoforms, pseudogenes, and antibody choice as well as primer design. Critical examination of the OCT- 4 literature leads us to suggest that OCT- 4 expression in fetal MSC may be a case of "The Emperor's New Clothes” with early reports of (false) positive expression amplified in subsequent studies without critical attention to emerging refinements in knowledge and methodology. Springer Science+Business Media, LLC, 2011 Mesenchymal stem cells nationallicence MSC nationallicence Fetal-placental tissue nationallicence Pluripotency nationallicence OCT- 4 nationallicence Stemness nationallicence Ryan Jennifer UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut Pettit Allison UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut Guillot Pascale Fetal Stem Cell Therapy Group, Institute of Reproductive & Developmental Biology, Imperial College, W12 ONN, London, United Kingdom aut Chan Jerry UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut Fisk Nicholas UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 408-421 1550-8943 9:4<408 2013 9 12015 https://doi.org/10.1007/s12015-011-9336-5 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-011-9336-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ryan Jennifer UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut NATIONALLICENCE 700 1- Pettit Allison UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut NATIONALLICENCE 700 1- Guillot Pascale Fetal Stem Cell Therapy Group, Institute of Reproductive & Developmental Biology, Imperial College, W12 ONN, London, United Kingdom aut NATIONALLICENCE 700 1- Chan Jerry UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut NATIONALLICENCE 700 1- Fisk Nicholas UQ Centre for Clinical Research, University of Queensland, Herston campus, 4029, Brisbane, Australia aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 408-421 1550-8943 9:4<408 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer