naa a22 4500 510739385 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12015-012-9355-x doi (NATIONALLICENCE)springer-10.1007/s12015-012-9355-x Epigenetic Modifications and Chromosome Conformations of the Beta Globin Locus throughout Development [Elektronische Daten] [Kai-Hsin Chang, Xiangdong Fang, Hao Wang, Andy Huang, Hua Cao, Yadong Yang, Halvard Bonig, John Stamatoyannopoulos, Thalia Papayannopoulou] Human embryonic stem cells provide an alternative to using human embryos for studying developmentally regulated gene expression. The co-expression of high levels of embryonic ε and fetal γ globin by the hESC-derived erythroblasts allows the interrogation of ε globin regulation at the transcriptional and epigenetic level which could only be attained previously by studying cell lines or transgenic mice. In this study, we compared the histone modifications across the β globin locus of the undifferentiated hESCs and hESC-, FL-, and mobilized PB CD34+ cells-derived erythroblasts, which have distinct globin expression patterns corresponding to their developmental stages. We demonstrated that the histone codes employed by the β globin locus are conserved throughout development. Furthermore, in spite of the close proximity of the ε globin promoter, as compared to the β or γ globin promoter, with the LCR, a chromatin loop was also formed between the LCR and the active ε globin promoter, similar to the loop that forms between the β or γ globin promoters and the LCR, in contrary to the previously proposed tracking mechanism. Springer Science+Business Media, LLC, 2012 Human embryonic stem cells nationallicence Erythroid cells nationallicence Fetal liver nationallicence Peripheral blood nationallicence Erythroblasts nationallicence Hemoglobin nationallicence Epigenetics nationallicence Histone modifications nationallicence Chromatin conformation nationallicence Chang Kai-Hsin Department of Medicine, Division of Hematology, University of Washington, NE Pacific St, Box 357710, 98195, Seattle, WA, USA aut Fang Xiangdong Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, China aut Wang Hao Department of Genome Sciences, University of Washington, 98195, Seattle, WA, USA aut Huang Andy Department of Medicine, Division of Medical Genetics, University of Washington, 98195, Seattle, WA, USA aut Cao Hua Department of Medicine, Division of Medical Genetics, University of Washington, 98195, Seattle, WA, USA aut Yang Yadong Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, China aut Bonig Halvard Department of Cellular Therapeutics/Cell Processing (GMP), German Red Cross Blood Service, 60528, Frankfurt, Germany aut Stamatoyannopoulos John Department of Genome Sciences, University of Washington, 98195, Seattle, WA, USA aut Papayannopoulou Thalia Department of Medicine, Division of Hematology, University of Washington, NE Pacific St, Box 357710, 98195, Seattle, WA, USA aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 397-407 1550-8943 9:4<397 2013 9 12015 https://doi.org/10.1007/s12015-012-9355-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9355-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Kai-Hsin Department of Medicine, Division of Hematology, University of Washington, NE Pacific St, Box 357710, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Fang Xiangdong Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, China aut NATIONALLICENCE 700 1- Wang Hao Department of Genome Sciences, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Huang Andy Department of Medicine, Division of Medical Genetics, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Cao Hua Department of Medicine, Division of Medical Genetics, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Yang Yadong Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, China aut NATIONALLICENCE 700 1- Bonig Halvard Department of Cellular Therapeutics/Cell Processing (GMP), German Red Cross Blood Service, 60528, Frankfurt, Germany aut NATIONALLICENCE 700 1- Stamatoyannopoulos John Department of Genome Sciences, University of Washington, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Papayannopoulou Thalia Department of Medicine, Division of Hematology, University of Washington, NE Pacific St, Box 357710, 98195, Seattle, WA, USA aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/4(2013-08-01), 397-407 1550-8943 9:4<397 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer