naa a22 4500 510739407 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12015-013-9452-5 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9452-5 Suppression of malignancy by Smad3 in mouse embryonic stem cell formed teratoma [Elektronische Daten] [Peng Li, Ying Chen, Meng Xiaoming, Ka Kwok, Xiaoru Huang, Kwong Choy, Chi Wang, Huiyao Lan, Ping Yuan] Disease associated gene deficient embryonic stem cells can serve as valuable in vitro models to study disease mechanisms and screen drugs. Smad3 mediated TGF-β/Activin/Nodal signaling plays important roles in many biological processes. Despite numerous studies regarding Smad3 function, the role of Smad3 in mouse ES cells is not well studied. To understand the function of Smad3 in mouse ES cells, we derived Smad3−/− ES cells and wild type ES cells. Smad3−/− ES cells display no defect on self-renewal. They express similar level of pluripotent genes and lineage genes compared to wild type ES cells. However, Smad3 ablation results in transient difference in germ layer marker expression during embryoid body formation. Mesoderm lineage marker expression is significantly reduced in the embryoid body formed by Smad3−/− ES cells compared to wild type ES cells. Intriguingly, subcutaneous injection of Smad3−/− ES cells into nude mice leads to formation of malignant immature teratomas, whilst wild type ES cells tend to form mature teratomas. Smad3−/− ES cell formed teratomas can therefore provide a new model for the study of the mechanism of malignant teratomas. Springer Science+Business Media New York, 2013 Embryonic stem cells nationallicence Smad3 nationallicence Teratoma nationallicence Li Peng Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Chen Ying Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Xiaoming Meng Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Kwok Ka Fetal Medicine Unit, Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Huang Xiaoru Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Choy Kwong Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Wang Chi Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Lan Huiyao Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Yuan Ping Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 709-720 1550-8943 9:5<709 2013 9 12015 https://doi.org/10.1007/s12015-013-9452-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9452-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Peng Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Chen Ying Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Xiaoming Meng Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Kwok Ka Fetal Medicine Unit, Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Huang Xiaoru Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Choy Kwong Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Wang Chi Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Lan Huiyao Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 700 1- Yuan Ping Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 709-720 1550-8943 9:5<709 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer