naa a22 4500 510739423 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12015-013-9440-9 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9440-9 Optimised Protocols for the Identification of the Murine Cardiac Side Population [Elektronische Daten] [Annette Meeson, Andrew Fuller, David Breault, W. Owens, Gavin Richardson] Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype relies on an active metabolic pathway and cell viability to identify a rare population of cells and therefore technical differences in the CSP staining protocol can lead to inconsistent results and discrepancies between studies. Here we describe an established protocol for CSP identification and have optimised a protocol for CSP analysis utilizing an automated cardiac digestion procedure using gentleMACs dissociation and Hoechst 33342 staining followed by dual wavelength flow cytometric analysis. Springer Science+Business Media New York, 2013 Cardiac nationallicence Side population nationallicence Abcg2 nationallicence Fumitremorgin C nationallicence Stem cell nationallicence FACS : Fluorescence-activated cell sorting nationallicence SP : Side population nationallicence CSP : Cardiac side population nationallicence FTC : Fumitremorgin C nationallicence Meeson Annette Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut Fuller Andrew Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut Breault David Division of Endocrinology, Children's Hospital Boston, Harvard Medical School, 02115, Boston, MA, USA aut Owens W. Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut Richardson Gavin Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 731-739 1550-8943 9:5<731 2013 9 12015 https://doi.org/10.1007/s12015-013-9440-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9440-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meeson Annette Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Fuller Andrew Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Breault David Division of Endocrinology, Children's Hospital Boston, Harvard Medical School, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Owens W. Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Richardson Gavin Institute of Genetic Medicine, International Centre for Life, Newcastle University, NE1 3BZ, Newcastle upon Tyne, UK aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 731-739 1550-8943 9:5<731 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer