naa a22 4500 510739431 CHVBK 20180411083022.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12015-013-9447-2 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9447-2 Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells [Elektronische Daten] [Betty Lawton, Julie Sosa, Sanziana Roman, Diane Krause] Definitive endoderm can be derived from human embryonic stem cells using low serum medium with cytokines involved in the epithelial-to-mesenchymal transition, including Activin A and Wnt3A. The purpose of this study was to develop an improved protocol that permits the induction of definitive endoderm while avoiding the high rate of cell death that often occurs with existing protocols. By including insulin and other nutrients, we demonstrate that cell viability can be preserved throughout differentiation. In addition, modifying a matrigel sandwich method previously reported to induce precardiac mesoderm allows for enhanced endodermal differentiation based on expression of endoderm-associated genes. The morphological and migratory characteristics of cells cultured by the technique, as well as gene expression patterns, indicate that the protocol can emulate key events in gastrulation towards the induction of definitive endoderm. Springer Science+Business Media New York, 2013 Matrigel nationallicence Endoderm nationallicence Epithelial nationallicence Mesenchymal nationallicence Stem cells nationallicence Lawton Betty Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA aut Sosa Julie Section of Endocrine Surgery, Department of Surgery, Duke University School of Medicine, Durham, NC, USA aut Roman Sanziana Section of Endocrine Surgery, Department of Surgery, Duke University School of Medicine, Durham, NC, USA aut Krause Diane Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 578-585 1550-8943 9:5<578 2013 9 12015 https://doi.org/10.1007/s12015-013-9447-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9447-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lawton Betty Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA aut NATIONALLICENCE 700 1- Sosa Julie Section of Endocrine Surgery, Department of Surgery, Duke University School of Medicine, Durham, NC, USA aut NATIONALLICENCE 700 1- Roman Sanziana Section of Endocrine Surgery, Department of Surgery, Duke University School of Medicine, Durham, NC, USA aut NATIONALLICENCE 700 1- Krause Diane Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 578-585 1550-8943 9:5<578 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer