naa a22 4500 510739458 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12015-013-9439-2 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9439-2 Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells [Elektronische Daten] [Jianyu Wang, Qi Gu, Jie Hao, Yundan Jia, Binghua Xue, Honghong Jin, Jing Ma, Renyue Wei, Tang Hai, Qingran Kong, Gerelchimeg Bou, Ping Xia, Qi Zhou, Liu Wang, Zhonghua Liu] Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with Oct4 (Pou5f1), Sox2, Klf4 and c-Myc (termed OSKM, 4F). Here, we found two other factors (Tbx3 and Nr5α2, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6F) orientated to mouse ESCs-like. Surprisingly, Nr5α2 alone could induce piPSCs formation in the presence or absence of c-Myc. These results suggested that Tbx3 and Nr5α2 may have vital roles in Sus scrofa and proposed new insights into pig pluripotent stem cells. Springer Science+Business Media New York, 2013 piPSCs nationallicence Nr5α2 nationallicence Tbx3 nationallicence Pluripotency nationallicence Efficiency nationallicence Wang Jianyu College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Gu Qi College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Hao Jie State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Jia Yundan State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Xue Binghua College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Jin Honghong College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Ma Jing College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Wei Renyue College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Hai Tang State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Kong Qingran College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Bou Gerelchimeg College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Xia Ping College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Zhou Qi State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Wang Liu State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Liu Zhonghua College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 700-708 1550-8943 9:5<700 2013 9 12015 https://doi.org/10.1007/s12015-013-9439-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9439-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jianyu College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Gu Qi College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Hao Jie State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Jia Yundan State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Xue Binghua College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Jin Honghong College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Ma Jing College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Wei Renyue College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Hai Tang State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Kong Qingran College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Bou Gerelchimeg College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Xia Ping College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 700 1- Zhou Qi State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Wang Liu State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Liu Zhonghua College of Life Science, Northeast Agricultural University of China, 150030, Harbin, People's Republic China aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 700-708 1550-8943 9:5<700 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer