naa a22 4500 510739466 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12015-013-9453-4 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9453-4 Searching for Prostate Cancer Stem Cells: Markers and Methods [Elektronische Daten] [Benjamin Sharpe, Mark Beresford, Rebecca Bowen, John Mitchard, Andrew Chalmers] The cancer stem cell hypothesis postulates that a single stem-like cancer cell is able to produce all cancer cell types found in a tumor. These cells are also thought to be the causative agents of relapse following therapy. In order to confirm the importance of cancer stem cells in tumor formation and patient prognosis, their role in prostate cancer must be comprehensively studied. This review describes current methods and markers for isolating and characterizing prostate cancer stem cells, including assays for self-renewal, multipotency and resistance to therapy. In particular the advantages and limitations of these approaches are analyzed. The review will also examine novel methods for studying the lineage of cancer stem cells in vivo using transgenic mouse models. These lineage tracing approaches have significant advantages and, if a number of challenges can be addressed, offer great potential for understanding the significance of cancer stem cells in human prostate cancer. Springer Science+Business Media New York, 2013 Biomarker nationallicence Prostate cancer nationallicence Cancer stem cell nationallicence Methods nationallicence Self-Renewal nationallicence Lineage tracing nationallicence Sharpe Benjamin Department of Biology and Biochemistry, University of Bath, BA2 7AY, Bath, UK aut Beresford Mark Department of Oncology, Royal United Hospital NHS Trust, BA1 3NG, Bath, UK aut Bowen Rebecca Department of Oncology, Royal United Hospital NHS Trust, BA1 3NG, Bath, UK aut Mitchard John Department of Pathology, Royal United Hospital NHS Trust, BA1 3NG, Bath, UK aut Chalmers Andrew Department of Biology and Biochemistry, University of Bath, BA2 7AY, Bath, UK aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 721-730 1550-8943 9:5<721 2013 9 12015 https://doi.org/10.1007/s12015-013-9453-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9453-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharpe Benjamin Department of Biology and Biochemistry, University of Bath, BA2 7AY, Bath, UK aut NATIONALLICENCE 700 1- Beresford Mark Department of Oncology, Royal United Hospital NHS Trust, BA1 3NG, Bath, UK aut NATIONALLICENCE 700 1- Bowen Rebecca Department of Oncology, Royal United Hospital NHS Trust, BA1 3NG, Bath, UK aut NATIONALLICENCE 700 1- Mitchard John Department of Pathology, Royal United Hospital NHS Trust, BA1 3NG, Bath, UK aut NATIONALLICENCE 700 1- Chalmers Andrew Department of Biology and Biochemistry, University of Bath, BA2 7AY, Bath, UK aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 721-730 1550-8943 9:5<721 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer