naa a22 4500 510739555 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12015-013-9446-3 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9446-3 Low Oxygen Tension is Critical for the Culture of Human Mesenchymal Stem Cells with Strong Osteogenic Potential from Haemarthrosis Fluid [Elektronische Daten] [Callie Knuth, Marcia Clark, Annette Meeson, Sameer Khan, Daniel Dowen, David Deehan, Rachel Oldershaw] Satisfactory osseous tissue integration of the soft tissue graft with bone is the mainstay of healing following surgical reconstruction of the anterior cruciate ligament (ACL). However, tissue remodelling is slow and significantly impacts on quality of life by delaying return to work and sport and accelerating the onset of degenerative diseases such as osteoarthritis. Delivery of multipotent human mesenchymal stem cells (hMSCs) at surgery could enhance osseous tissue integration. We aim to use hMSCs derived from haemarthrosis fluid (HF) (the intra-articular bleed accrued post-trauma) which is aspirated and discarded as clinical waste. With the aim of improving our bioprocessing methodologies for clinical translation we have investigated the effect of low oxygen tension on the derivation and osteogenic potential of this novel HF-hMSC population. Mononuclear cells were isolated from HF aspirated samples and divided for derivation and culture under normal or low oxygen tension. HF-hMSCs were derived from 100% of cultures under low oxygen tension compared to 71% for normal oxygen tension; this was coupled with increased CFU-Fs. We investigated the osteogenic potential and cellular health of HF-hMSC populations following ex vivo expansion. HF-hMSC populations showed enhanced matrix mineralisation and cellular health when differentiated under low oxygen tension. This positive effect of low oxygen on osteogenesis and cellular health was reduced with prolonged culture. These data demonstrate that derivation and culture of HF-hMSC populations under low oxygen tension will enable the translation of a cellular therapy for the treatment of broad patient numbers with optimal osteogenic potency and cellular vitality. Springer Science+Business Media New York, 2013 Haemarthrosis nationallicence Mesenchymal stem cell nationallicence Anterior cruciate ligament nationallicence Low oxygen tension nationallicence Knuth Callie Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut Clark Marcia Freeman Hospital, Newcastle-Upon-Tyne NHS Trust, Freeman Road, NE7 7DN, Newcastle-upon-Tyne, High Heaton, UK aut Meeson Annette Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut Khan Sameer Freeman Hospital, Newcastle-Upon-Tyne NHS Trust, Freeman Road, NE7 7DN, Newcastle-upon-Tyne, High Heaton, UK aut Dowen Daniel Freeman Hospital, Newcastle-Upon-Tyne NHS Trust, Freeman Road, NE7 7DN, Newcastle-upon-Tyne, High Heaton, UK aut Deehan David Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut Oldershaw Rachel Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 599-608 1550-8943 9:5<599 2013 9 12015 https://doi.org/10.1007/s12015-013-9446-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9446-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Knuth Callie Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut NATIONALLICENCE 700 1- Clark Marcia Freeman Hospital, Newcastle-Upon-Tyne NHS Trust, Freeman Road, NE7 7DN, Newcastle-upon-Tyne, High Heaton, UK aut NATIONALLICENCE 700 1- Meeson Annette Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut NATIONALLICENCE 700 1- Khan Sameer Freeman Hospital, Newcastle-Upon-Tyne NHS Trust, Freeman Road, NE7 7DN, Newcastle-upon-Tyne, High Heaton, UK aut NATIONALLICENCE 700 1- Dowen Daniel Freeman Hospital, Newcastle-Upon-Tyne NHS Trust, Freeman Road, NE7 7DN, Newcastle-upon-Tyne, High Heaton, UK aut NATIONALLICENCE 700 1- Deehan David Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut NATIONALLICENCE 700 1- Oldershaw Rachel Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, International Centre for Life, NE1 4EP, Newcastle-upon-Tyne, Times Square, UK aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Springer US; http://www.springer-ny.com 9/5(2013-10-01), 599-608 1550-8943 9:5<599 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer