naa a22 4500 510739601 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12015-012-9422-3 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9422-3 Treatment Efficacy with Bone Marrow Derived Mesenchymal Stem Cells and Minocycline in Rats After Cerebral Ischemic Injury [Elektronische Daten] [Sule Bilen, Ferda Pinarli, Fikri Ak, Ersin Fadillioglu, Aynur Albayrak, Gulbahar Boyuk, Osman Guler, Gonul Erden, A. Ulus, Tuncay Delibasi] Objective: We aimed to investigate the effects of bone marrow derived mesenchymal stem cells (MSCs), minocycline, and these two therapies combined on functional and histological improvement in cerebral ischemic injury created rats. Materials and methods: Twenty-eight Sprague Dawley female rats, weighing 250-300g, were included in the study. Two male rats with similar properties were sacrificed for bone marrow derived MSC production. Group 1 was established as the control group. Group 2 was the group of only minocycline administered rats. Group 3 was the one of only MSCs administered rats. Group 4 was composed of the rats given the combination of MSCs and minocycline. Hematoxylin and eosin staining was done to assess the degeneration of the cells. Immunohistochemical staining was performed to evaluate the regeneration. Motor functions were examined by using Bederson's score. Results: Cell degeneration was the least in group 4. The cells stained with GFAP were observed mostly in group 4. The cells stained with Neu N in group 1 were statistically lower than in other groups. When the groups were ordered in terms of functional improvement at the end of the second week, group 4 had the most and group 1 had the least. Conclusions: Bone marrow derived MSCs can lead to more histological and functional improvement when administered with minocycline, which is a neuroprotective agent as early as 24h following the ischemic injury in a rat model. Minocycline therapy alone can be as effective as bone marrow derived MSCs therapy alone in ischemic cerebral rat model. Springer Science+Business Media New York, 2012 Cerebral ischemic injury nationallicence Bone marrow derived mesenchymal stem cell nationallicence Minocycline nationallicence Functional improvement nationallicence Bilen Sule Department of Neurology, Ankara Numune Education and Research Hospital, Ankara, Turkey aut Pinarli Ferda Medical Research Center of Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut Ak Fikri Department of Neurology, Ankara Numune Education and Research Hospital, Ankara, Turkey aut Fadillioglu Ersin Department of Physiology, Hacettepe University Faculty of Medicine, Ankara, Turkey aut Albayrak Aynur Department of Pathology, Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut Boyuk Gulbahar Medical Research Center of Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut Guler Osman Medical Research Center of Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut Erden Gonul Department of Biochemistry, Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut Ulus A. Department of Cardiovascular Surgery, Ankara Yuksek Ihtisas Education and Research Hospital, Ankara, Turkey aut Delibasi Tuncay Department of Endocrinology and Metabolism, Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 219-225 1550-8943 9:2<219 2013 9 12015 https://doi.org/10.1007/s12015-012-9422-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9422-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bilen Sule Department of Neurology, Ankara Numune Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Pinarli Ferda Medical Research Center of Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Ak Fikri Department of Neurology, Ankara Numune Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Fadillioglu Ersin Department of Physiology, Hacettepe University Faculty of Medicine, Ankara, Turkey aut NATIONALLICENCE 700 1- Albayrak Aynur Department of Pathology, Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Boyuk Gulbahar Medical Research Center of Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Guler Osman Medical Research Center of Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Erden Gonul Department of Biochemistry, Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Ulus A. Department of Cardiovascular Surgery, Ankara Yuksek Ihtisas Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Delibasi Tuncay Department of Endocrinology and Metabolism, Ankara Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 219-225 1550-8943 9:2<219 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer