naa a22 4500 510739644 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12015-013-9426-7 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9426-7 Human Wharton's Jelly Stem Cell Conditioned Medium Enhances Freeze-Thaw Survival and Expansion of Cryopreserved CD34+ Cells [Elektronische Daten] [Hao Lin, Ariff Bongso, Kalamegam Gauthaman, Arijit Biswas, Mahesh Choolani, Chui-Yee Fong] Hematopoietic stem cells (HSCs) from umbilical cord blood have been successfully used to treat blood disorders but one major hurdle is the relatively low cell dose available. Double cord blood unit transplantation results in elevated engraftment failure because one unit predominates over the other. Various approaches are thus being undertaken to expand HSCs ex vivo from single cord blood units. We report here a protocol involving slow freezing (−1°C per minute to −120°C) + freezing medium containing DMSO + FBS + 24h-50% hWJSC-CM that enhances thaw-survival of CD34+ cells. Post-thawing, the fold, percentage and colony forming unit numbers of CD34+ cells were significantly increased (2.08 ± 0.3; 102 ± 1.17%; 1.07 ± 0.02 respectively) while the percentages of apoptotic, necrotic, dead and sub-G1 phase cells (91.06 ± 3.63%; 91.80 ± 5.01%; 95.6 ± 3.61%; 86.1 ± 16.26% respectively) were significantly decreased compared to controls. Post-thaw culture in 24h-50% hWJSC-CM+FBS for 72h showed further significant increases in CD34+ cells (fold: 2.28 ± 0.17; percentage: 153.3 ± 21.99%, CFU: 1.6 ± 0.19) and significant decreases in apoptotic, necrotic, dead and sub-G1 cells (49.2 ± 3.59%; 62.0 ± 4.30%; 56.6 ± 5.06%; 28.6 ± 5.74% respectively) compared to controls. We hypothesize that these improvements are probably related to the high levels of cytokines, cell adhesion molecules and growth factors in hWJSC-CM that help to preserve cell membrane integrity during freezing and stimulate mitosis post-thaw. A 24h-50% hJWSC-CM may be a useful supplement for freezing CD34+ cells in cord blood banks. Springer Science+Business Media New York, 2013 Cord blood banks nationallicence Expansion of human umbilical cord blood CD34+ cells nationallicence Human Wharton's jelly stem cell conditioned medium nationallicence Slow freezing nationallicence Lin Hao Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut Bongso Ariff Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut Gauthaman Kalamegam Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut Biswas Arijit Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut Choolani Mahesh Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut Fong Chui-Yee Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 172-183 1550-8943 9:2<172 2013 9 12015 https://doi.org/10.1007/s12015-013-9426-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9426-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Hao Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut NATIONALLICENCE 700 1- Bongso Ariff Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut NATIONALLICENCE 700 1- Gauthaman Kalamegam Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut NATIONALLICENCE 700 1- Biswas Arijit Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut NATIONALLICENCE 700 1- Choolani Mahesh Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut NATIONALLICENCE 700 1- Fong Chui-Yee Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University Health System, 119228, Kent Ridge, Singapore aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 172-183 1550-8943 9:2<172 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer