naa a22 4500 510739679 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12015-012-9417-0 doi (NATIONALLICENCE)springer-10.1007/s12015-012-9417-0 Phenol Red Inhibits Chondrogenic Differentiation and Affects Osteogenic Differentiation of Human Mesenchymal Stem Cells in Vitro [Elektronische Daten] [Helle Lysdahl, Anette Baatrup, Anna Nielsen, Casper Foldager, Cody Bünger] The purpose with this study was to investigate the effect of phenol red (PR) on chondrogenic and osteogenic differentiation of human mesenchymal stem cells (hMSCs). hMSCs were differentiated into chondrogenic and osteogenic directions in DMEM with and without PR for 2, 7, 14, 21, and 28days. Gene expression of chondrogenic and osteogenic markers were analyzed by RT-qPCR. The presence of proteoglycans was visualized histologically. Osteogenic matrix deposition and mineralization were examined measuring the alkaline phophatase activity and calcium deposition. During chondrogenic differentiation PR decreased sox9, collagen type 2, aggrecan on day 14 and 21 (P < 0.05), and proteoglycan synthesis on day 21 and 28. Collagen type 10 was decreased on day 21 (P < 0.05). During osteogenic differentiation PR increased alkaline phosphatase on day 7 while decreased on day 21 (P < 0.05). PR increased collagen type 1 on day 7, 14, and day 21 (P < 0.05). The alkaline phosphatase activity was increased after 2, 7, and 14days (P < 0.05). The deposition of calcium was decreased on day 21 (P < 0.05). Our results indicate that PR should be removed from the culture media when differentiating hMSCs into chondrogenic and osteogenic directions due to the effects on these differentiation pathways. Springer Science+Business Media New York, 2012 Human mesenchymal stem cells nationallicence Culture media nationallicence Phenol red nationallicence Chondrogenic differentiation nationallicence Osteogenic differentiation nationallicence Lysdahl Helle Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut Baatrup Anette Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut Nielsen Anna Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut Foldager Casper Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut Bünger Cody Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 132-139 1550-8943 9:2<132 2013 9 12015 https://doi.org/10.1007/s12015-012-9417-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-012-9417-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lysdahl Helle Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Baatrup Anette Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Nielsen Anna Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Foldager Casper Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut NATIONALLICENCE 700 1- Bünger Cody Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, 8000, Aarhus C, Denmark aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 132-139 1550-8943 9:2<132 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer