naa a22 4500 510739695 CHVBK 20180411083023.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12015-013-9428-5 doi (NATIONALLICENCE)springer-10.1007/s12015-013-9428-5 Thermoresponsive Substrates used for the Expansion of Human Mesenchymal Stem Cells and the Preservation of Immunophenotype [Elektronische Daten] [Maria Nash, Xingliang Fan, William Carroll, Alexander Gorelov, Frank Barry, Georgina Shaw, Yury Rochev] The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercially available pNIPAm which is both affordable and a significant simplification on alternative approaches used elsewhere. Solvent casting was used to produce films in the micrometer range and successful cell adhesion and proliferation was highly dependent on the thickness of the coating produced with 1μm thick coatings supporting cells to confluence. 3T3 cell sheets and hMSCs were successfully detached from the cast coatings upon temperature reduction. Furthermore, results indicate that the hMSCs remained undifferentiated as the surface receptor profile of hMSCs was not altered when cells were detached in this manner. Springer Science+Business Media New York, 2013 Thermoresponsive polymers nationallicence Human mesenchymal stem cells nationallicence Cell expansion and regeneration nationallicence Flow cytometry nationallicence Stem cell differentiation nationallicence Nash Maria Department of Applied Macromolecular Chemistry, Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006, Madrid, Spain aut Fan Xingliang National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland aut Carroll William School of Chemistry, National University of Ireland, Galway, Ireland aut Gorelov Alexander School of Chemistry and Chemical Biology, University College Dublin, Dublin, Ireland aut Barry Frank Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland aut Shaw Georgina Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland aut Rochev Yury School of Chemistry, National University of Ireland, Galway, Ireland aut Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 148-157 1550-8943 9:2<148 2013 9 12015 https://doi.org/10.1007/s12015-013-9428-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12015-013-9428-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nash Maria Department of Applied Macromolecular Chemistry, Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006, Madrid, Spain aut NATIONALLICENCE 700 1- Fan Xingliang National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland aut NATIONALLICENCE 700 1- Carroll William School of Chemistry, National University of Ireland, Galway, Ireland aut NATIONALLICENCE 700 1- Gorelov Alexander School of Chemistry and Chemical Biology, University College Dublin, Dublin, Ireland aut NATIONALLICENCE 700 1- Barry Frank Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland aut NATIONALLICENCE 700 1- Shaw Georgina Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland aut NATIONALLICENCE 700 1- Rochev Yury School of Chemistry, National University of Ireland, Galway, Ireland aut NATIONALLICENCE 773 0- Stem Cell Reviews and Reports Humana Press Inc 9/2(2013-04-01), 148-157 1550-8943 9:2<148 2013 9 12015 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer