naa a22 4500 510741266 CHVBK 20180411083029.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11845-013-0941-y doi (NATIONALLICENCE)springer-10.1007/s11845-013-0941-y Tumor suppressive miR-509-5p contributes to cell migration, proliferation and antiapoptosis in renal cell carcinoma [Elektronische Daten] [W. Zhang, Z. Pan, Q. Yang, X. Zheng] Purpose: The aim of this study was to determine the expression and function of miR-509-5p in renal cell carcinoma (RCC). Materials and methods: In this research, we have conducted quantitative real-time polymerase chain reaction (qRT-PCR) assay to determine the expression level of miR-509-5p in tissues and plasma from renal cell carcinoma patients. We preformed in vitro migration scratch assay, flow cytometry analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the exact function of miR-509-5p. Results: We evaluated the expression level of miR-509-5p in RCC tissues and paired adjacent normal tissues from 42 patients and found that miR-509-5p expression in 42 RCC specimens was significantly down-regulated compared to that in adjacent normal tissue. Furthermore, the level of miR-509-5p in RCC patients' plasma was significantly lower than that in control plasma. In addition, the overexpression of miR-509-5p suppressed the proliferation of RCC cell (786-0), induced cell apoptosis and inhibited cell migration in vitro. Conclusion: In this study, we have shown that miR-509-5p played an important role in RCC by inhibiting cell proliferation and migration and by promoting cell apoptosis. In addition, miR-509-5p expression was significantly lower in RCC patient plasma compared to that in normal individuals. Royal Academy of Medicine in Ireland, 2013 miR-509-5p nationallicence Renal cell carcinoma nationallicence MicroRNA nationallicence Kidney cancer nationallicence Apoptosis nationallicence Migration nationallicence Zhang W. Department of Urology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuhan, China aut Pan Z. Department of Emergency, Zhongnan Hospital, Wuhan University, Wuhan, China aut Yang Q. Department of Emergency, Zhongnan Hospital, Wuhan University, Wuhan, China aut Zheng X. Department of Urology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuhan, China aut Irish Journal of Medical Science Springer London 182/4(2013-12-01), 621-627 0021-1265 182:4<621 2013 182 11845 https://doi.org/10.1007/s11845-013-0941-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11845-013-0941-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang W. Department of Urology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuhan, China aut NATIONALLICENCE 700 1- Pan Z. Department of Emergency, Zhongnan Hospital, Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Yang Q. Department of Emergency, Zhongnan Hospital, Wuhan University, Wuhan, China aut NATIONALLICENCE 700 1- Zheng X. Department of Urology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuhan, China aut NATIONALLICENCE 773 0- Irish Journal of Medical Science Springer London 182/4(2013-12-01), 621-627 0021-1265 182:4<621 2013 182 11845 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer