naa a22 4500 510745113 CHVBK 20180411083043.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s13364-013-0146-9 doi (NATIONALLICENCE)springer-10.1007/s13364-013-0146-9 Fast and efficient DNA-based method for winter diet analysis from stools of three cervids: moose, red deer, and roe deer [Elektronische Daten] [Marta Czernik, Pierre Taberlet, Magdalena Świsłocka, Magdalena Czajkowska, Norbert Duda, Mirosław Ratkiewicz] Effects of cervid browsing on timber production, especially during winter, lead to economic losses in forest management. The aim of this study was to present an efficient DNA-based method which allows qualitative assessment of the winter diet from stools of moose (Alces alces), roe deer (Capreolus capreolus), and red deer (Cervus elaphus). The preliminary results of the diet composition of the three cervids from Poland were also presented with a special emphasis on moose. The electropherograms of the chloroplast intron trnL (UAA) P6 loop amplification products using g (fluorescence-labeled) and h primers revealed differences in the length of PCR products among various plant species eaten by these herbivores. In addition, the usage of species-specific primers allowed unambiguous identification of different gymnosperms and angiosperms. The preliminary moose diet analysis, based on winter fecal samples from the entire range of moose occurrence in Poland, revealed the presence of 15 to 24 tree, shrub, and herbaceous species. This fast, cost-efficient, and simple method proved also to be reliable for the diet analysis of red deer and roe deer. It may be a valuable tool in forest and conservation management, as well as a way of enhancing ecological studies focusing on the impact of herbivores on the ecosystems and their possible food niche overlap. The Author(s), 2013 Alces alces nationallicence Capreolus capreolus nationallicence Cervus elaphus nationallicence DNA metabarcoding nationallicence Ungulate diet analysis nationallicence Ungulate management nationallicence Czernik Marta Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut Taberlet Pierre Laboratoire d'Ecologie Alpine, CNRS, UMR 5553, Université Joseph Fourier, 2233 Rue de la Piscine, 38041, Grenoble Cedex 9, France aut Świsłocka Magdalena Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut Czajkowska Magdalena Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut Duda Norbert Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut Ratkiewicz Mirosław Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut Acta Theriologica Springer Berlin Heidelberg 58/4(2013-10-01), 379-386 0001-7051 58:4<379 2013 58 13364 https://doi.org/10.1007/s13364-013-0146-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13364-013-0146-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Czernik Marta Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut NATIONALLICENCE 700 1- Taberlet Pierre Laboratoire d'Ecologie Alpine, CNRS, UMR 5553, Université Joseph Fourier, 2233 Rue de la Piscine, 38041, Grenoble Cedex 9, France aut NATIONALLICENCE 700 1- Świsłocka Magdalena Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut NATIONALLICENCE 700 1- Czajkowska Magdalena Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut NATIONALLICENCE 700 1- Duda Norbert Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut NATIONALLICENCE 700 1- Ratkiewicz Mirosław Institute of Biology, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland aut NATIONALLICENCE 773 0- Acta Theriologica Springer Berlin Heidelberg 58/4(2013-10-01), 379-386 0001-7051 58:4<379 2013 58 13364 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer