naa a22 4500 510745245 CHVBK 20180411083043.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s13364-012-0096-7 doi (NATIONALLICENCE)springer-10.1007/s13364-012-0096-7 Cytochrome b gene ( cytb ) sequence diversity in a Microtus oeconomus population from Bialowieza Primeval Forest [Elektronische Daten] [Michał Dąbrowski, Jan Pomorski, Joanna Gliwicz] Based on published information about the glacial, postglacial, and recent distribution of the root vole, Microtus oeconomus, we hypothesized that a population inhabiting the pristine wetland in eastern Poland (Bialowieza Primeval Forest) might comprise a high diversity of haplotypes. The support for this hypothesis was provided by an analysis of partial cytb gene sequences from 149 voles sampled within a two-hectare plot during a nine-year study. In this population, we identified eight haplotypes (PLB1-PLB8), four of which were new to the root vole. These haplotypes were characterized by low nucleotide diversity (π = 0.0054, SE = 0.0019), the absence of transversional differences between sequences, and no changes in the encoded amino acid sequence: features suggesting a lack of immigration from the distant populations. The haplotype number and their frequency distribution in males and females did not differ significantly. An assessment of the persistence of matrilines in the local population throughout the study period revealed that the haplotype composition was relatively stable for only about 3years. A more complete haplotype network for root voles in Europe was constructed by combining the newly identified haplotypes with the 45 previously described haplotypes. Two of the haplotypes detected in this study occupy key positions in this network: PLB5, as the closest link to the North European group, and PLB8, as an ancestor to many other Central European haplotypes. The Author(s), 2012 Bialowieza nationallicence Cytochrome b gene nationallicence Dispersal nationallicence Haplotype nationallicence Long-term study nationallicence Root vole nationallicence Dąbrowski Michał Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland aut Pomorski Jan Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland aut Gliwicz Joanna Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland aut Acta Theriologica Springer-Verlag 58/2(2013-04-01), 119-126 0001-7051 58:2<119 2013 58 13364 https://doi.org/10.1007/s13364-012-0096-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13364-012-0096-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dąbrowski Michał Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland aut NATIONALLICENCE 700 1- Pomorski Jan Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland aut NATIONALLICENCE 700 1- Gliwicz Joanna Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679, Warsaw, Poland aut NATIONALLICENCE 773 0- Acta Theriologica Springer-Verlag 58/2(2013-04-01), 119-126 0001-7051 58:2<119 2013 58 13364 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer