naa a22 4500 510745253 CHVBK 20180411083043.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s13364-012-0095-8 doi (NATIONALLICENCE)springer-10.1007/s13364-012-0095-8 Estimating the population structure of brown bears in eastern Hokkaido based on microsatellite analysis [Elektronische Daten] [Tetsuji Itoh, Yoshikazu Sato, Hifumi Tsuruga, Tsutomu Mano, Masao Kohira, Masami Yamanaka, Shinsuke Kasai, Kyoko Kobayashi, Ryûtarô Iwata] Estimating the genetic structure of a population is important for the conservation and management of wildlife. In the present study, our aim was to estimate the genetic structure of the brown bear (Ursus arctos) population in eastern Hokkaido by performing a Bayesian clustering analysis. To accomplish this goal, we used 15 microsatellites to generate genotypic data from tissue samples collected from 646 bears between 1996 and 2008. Using this genotypic data and the geographic locations where the bears were captured, GENELAND analysis detected six subpopulations. Based on the genotypic data, the STRUCTURE analysis revealed three subpopulations. As inferred from the GENELAND analysis, the core zones of the subpopulations (G-a through G-f) were located in the Shiranuka Hills (G-a), the northern area of the Shiranuka Hills (G-b), the eastern slope of the Daisetsuzan Mountains (G-c), the northern slope of the Akan Mountain Range (G-d), the Shiretoko Peninsula (G-e), and Akkeshi District (G-f). The STRUCTURE analysis indicated that G-b and G-d were influenced by gene flow from other subpopulations. National routes, towns, and farm fields were considered to have formed the distribution boundaries among the subpopulations. A high level of genetic differentiation was not observed among the six subpopulations, with the exception of G-f (F st = 1.35-0.176, D s = 0.246-0.349), which showed a geographically discontinuous distribution. We suggest that the loss of forest areas through future regional development and road building should be avoided to facilitate gene flow in brown bears in Hokkaido. Mammal Research Institute, Polish Academy of Sciences, Białowieża, Poland, 2012 Ursus arctos nationallicence Microsatellite nationallicence Bayesian clustering nationallicence Genetic differentiation nationallicence Genetic variation nationallicence Geographical barrier nationallicence Itoh Tetsuji Graduate School of Bioresource Sciences, Nihon University, Kameino 1866, 252-0880, Fujisawa, Kanagawa, Japan aut Sato Yoshikazu College of Bioresource Sciences, Nihon University, Kameino 1866, 252-0880, Fujisawa, Kanagawa, Japan aut Tsuruga Hifumi Hokkaido Institute of Environmental Sciences, 060-0819, Sapporo, Japan aut Mano Tsutomu Hokkaido Institute of Environmental Sciences, 060-0819, Sapporo, Japan aut Kohira Masao Shiretoko Nature Foundation, 531 Iwaobetsu, Shari, 099-4356, Hokkaido, Japan aut Yamanaka Masami Shiretoko Nature Foundation, 531 Iwaobetsu, Shari, 099-4356, Hokkaido, Japan aut Kasai Shinsuke Shiretoko Nature Foundation, 531 Iwaobetsu, Shari, 099-4356, Hokkaido, Japan aut Kobayashi Kyoko United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan aut Iwata Ryûtarô College of Bioresource Sciences, Nihon University, Kameino 1866, 252-0880, Fujisawa, Kanagawa, Japan aut Acta Theriologica Springer-Verlag 58/2(2013-04-01), 127-138 0001-7051 58:2<127 2013 58 13364 https://doi.org/10.1007/s13364-012-0095-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13364-012-0095-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Itoh Tetsuji Graduate School of Bioresource Sciences, Nihon University, Kameino 1866, 252-0880, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sato Yoshikazu College of Bioresource Sciences, Nihon University, Kameino 1866, 252-0880, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Tsuruga Hifumi Hokkaido Institute of Environmental Sciences, 060-0819, Sapporo, Japan aut NATIONALLICENCE 700 1- Mano Tsutomu Hokkaido Institute of Environmental Sciences, 060-0819, Sapporo, Japan aut NATIONALLICENCE 700 1- Kohira Masao Shiretoko Nature Foundation, 531 Iwaobetsu, Shari, 099-4356, Hokkaido, Japan aut NATIONALLICENCE 700 1- Yamanaka Masami Shiretoko Nature Foundation, 531 Iwaobetsu, Shari, 099-4356, Hokkaido, Japan aut NATIONALLICENCE 700 1- Kasai Shinsuke Shiretoko Nature Foundation, 531 Iwaobetsu, Shari, 099-4356, Hokkaido, Japan aut NATIONALLICENCE 700 1- Kobayashi Kyoko United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Iwata Ryûtarô College of Bioresource Sciences, Nihon University, Kameino 1866, 252-0880, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 773 0- Acta Theriologica Springer-Verlag 58/2(2013-04-01), 127-138 0001-7051 58:2<127 2013 58 13364 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer