naa a22 4500 510745733 CHVBK 20180411083045.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12192-012-0359-x doi (NATIONALLICENCE)springer-10.1007/s12192-012-0359-x Dynamics of endogenous Hsp70 synthesis in the brain of olfactory bulbectomized mice [Elektronische Daten] [Natalia Bobkova, Irina Guzhova, Boris Margulis, Inna Nesterova, Natalia Medvedinskaya, Alexander Samokhin, Irina Alexandrova, David Garbuz, Evgeny Nudler, Michael Evgen'ev] Numerous epidemiological studies have established acute brain injury as one of the major risk factors for the Alzheimer's disease (AD). However, the lack of animal models of AD-like degeneration triggered by a defined injury hampered the development of adequate therapies. Here we report that the surgical damage of the olfactory bulbs triggers the development of several pathologies, including amyloid-β accumulation and strong decrease of neuron density in the cortex and hippocampus as well as significant disturbance of spatial memory. Characteristically, these harmful consequences of the olfactory bulbectomy (OBX) have a peculiar dynamics in time with maximal manifestation in periods of 1-1.5months and 8months after the surgery and, hence, exhibit biphasic pattern with almost complete recovery period taking place at 5-6months after the operation. The quantitative determination of endogenous inducible form of Hsp70 in different brain areas of OBX mice demonstrated characteristic fluctuations of Hsp70 levels depending on the time after the operation and age of mice. Interestingly, maximal induction of Hsp70 synthesis in the hippocampus exhibits clear-cut coincidence with the recovery period in OBX animals. The observed correlation enables to suggest curing effect of Hsp70 synthesis at an earlier period of pathology development and establishes it as a possible therapeutic agent for secondary grave consequences of brain injury, such as AD-like degeneration, for which neuroprotective therapy is urgently needed. Cell Stress Society International, 2012 Olfactory bulbectomy nationallicence Spatial memory nationallicence Neurodegeneration nationallicence Hsp70 nationallicence Amyloid-β (Aβ) nationallicence Alzheimer's disease nationallicence Bobkova Natalia Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut Guzhova Irina Institute of Cytology, RAS, 194064, St. Petersburg, Russia aut Margulis Boris Institute of Cytology, RAS, 194064, St. Petersburg, Russia aut Nesterova Inna Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut Medvedinskaya Natalia Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut Samokhin Alexander Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut Alexandrova Irina Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut Garbuz David Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991, Moscow, Russia aut Nudler Evgeny Department of Biochemistry, New York University School of Medicine, 10016, New York, NY, USA aut Evgen'ev Michael Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 109-118 1355-8145 18:1<109 2013 18 12192 https://doi.org/10.1007/s12192-012-0359-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0359-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bobkova Natalia Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Guzhova Irina Institute of Cytology, RAS, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Margulis Boris Institute of Cytology, RAS, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Nesterova Inna Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Medvedinskaya Natalia Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Samokhin Alexander Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Alexandrova Irina Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 700 1- Garbuz David Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Nudler Evgeny Department of Biochemistry, New York University School of Medicine, 10016, New York, NY, USA aut NATIONALLICENCE 700 1- Evgen'ev Michael Institute of Cell Biophysics, RAS, 142290, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 109-118 1355-8145 18:1<109 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer