naa a22 4500 510745784 CHVBK 20180411083045.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12192-012-0362-2 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0362-2 Hyperbaric oxygen preconditioning protects skin from UV-A damage [Elektronische Daten] [Ashley Fuller, Charles Giardina, Lawrence Hightower, George Perdrizet, Cassandra Tierney] Hyperbaric oxygen therapy (HBOT) is used for a number of applications, including the treatment of diabetic foot ulcers and CO poisoning. However, we and others have shown that HBOT can mobilize cellular antioxidant defenses, suggesting that it may also be useful under circumstances in which tissue protection from oxidative damage is desired. To test the protective properties of hyperbaric oxygen (HBO) on a tissue level, we evaluated the ability of a preconditioning treatment regimen to protect cutaneous tissue from UV-A-induced oxidative damage. Three groups of hairless SKH1-E mice were exposed to UV-A 3days per week for 22weeks, with two of these groups receiving an HBO pretreatment either two or four times per week. UV-A exposure increased apoptosis and proliferation of the skin tissue, indicating elevated levels of epithelial damage and repair. Pretreatment with HBO significantly reduced UV-A-induced apoptosis and proliferation. A morphometric analysis of microscopic tissue folds also showed a significant increase in skin creasing following UV-A exposure, which was prevented by HBO pretreatment. Likewise, skin elasticity was found to be greatest in the group treated with HBO four times per week. The effects of HBO were also apparent systemically as reductions in caspase-3 activity and expression were observed in the liver. Our findings support a protective function of HBO pretreatment from a direct oxidative challenge of UV-A to skin tissue. Similar protection of other tissues may likewise be achievable. Cell Stress Society International, 2012 Hyperbaric oxygen nationallicence Preconditioning nationallicence UV-A nationallicence Skin nationallicence Apoptosis nationallicence Liver nationallicence SKH1-E mice nationallicence Fuller Ashley Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut Giardina Charles Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut Hightower Lawrence Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut Perdrizet George Wound Recovery and Hyperbaric Medicine Center, Kent Hospital, 02886, Warwick, RI, USA aut Tierney Cassandra Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 97-107 1355-8145 18:1<97 2013 18 12192 https://doi.org/10.1007/s12192-012-0362-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0362-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fuller Ashley Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Giardina Charles Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Hightower Lawrence Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Perdrizet George Wound Recovery and Hyperbaric Medicine Center, Kent Hospital, 02886, Warwick, RI, USA aut NATIONALLICENCE 700 1- Tierney Cassandra Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, U3125, 06269, Storrs, CT, USA aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 97-107 1355-8145 18:1<97 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer