naa a22 4500 510745806 CHVBK 20180411083045.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12192-012-0361-3 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0361-3 Recognition of self-heat shock protein 60 by T cells from patients with atopic dermatitis [Elektronische Daten] [Berber Kapitein, Joost Aalberse, Mark Klein, Wilco de Jager, Maarten Hoekstra, Edward Knol, Berent Prakken] Heat shock protein 60 (hsp60) is a highly conserved stress protein and target of self-reactive T cells in various inflammatory diseases. Not much is known about a possible role in atopic disease. As atopic diseases are considered to be the result of a disturbance in the balance between T helper cells type 2 and regulatory T cells, it is of interest to know whether hsp60 acts as a bystander antigen in atopic disease. Our aim was to investigate whether hsp60 is involved in the chronicity of inflammation of atopic dermatitis (AD). We studied the expression of hsp60 in skin tissue of adults with AD by immunohistochemistry. Peripheral blood mononuclear cells (PBMC) of children with AD were cultured with hsp60 and proliferative responses, cytokine secretion, surface markers, and functional assays were compared to responses of PBMC of healthy controls (HC). Hsp60 was detected more in lesional skin of AD patients compared to nonlesional skin. Furthermore, PBMC of children with AD proliferated more strongly in response to hsp60 compared to HC. hsp60-reactive T cells of atopic children produced high levels of IFNγ and low levels of IL-10. In vitro activation with hsp60 leads to the induction of CD4+CD25bright T cells expressing FOXP3 in both HC as well as in atopic children. However, despite their regulatory phenotype, hsp60-induced CD4+CD25brightCD127−FOXP3+ T cells of AD patients were incapable of suppressing effector T cells in vitro. hsp60 is recognized by proinflammatory (IFNγ high, IL-10 low) T cells in atopic patients and is more present in lesional AD skin. This suggests that hsp60-specific T cell responses contribute to local inflammation in AD. Cell Stress Society International, 2012 Atopy nationallicence Atopic dermatitis nationallicence Regulatory T cells nationallicence Human heat shock protein 60 nationallicence TH2 : T helper cells type 2 nationallicence TH1 : T helper cells type 1 nationallicence Tregs : Naturally occurring or induced CD4+CD25+FOXP3+ regulatory T cells nationallicence hsp : Heat shock protein(s) nationallicence hsp60 : Human heat shock protein 60 nationallicence PBMC : Peripheral blood mononuclear cells nationallicence AD : Atopic dermatitis nationallicence FACS : Fluorescence-activated cell sorting nationallicence Kapitein Berber Department of General Paediatrics, Wilhelmina Children's Hospital, Utrecht, Netherlands aut Aalberse Joost Department of General Paediatrics, Wilhelmina Children's Hospital, Utrecht, Netherlands aut Klein Mark Department of Paediatric Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, KC 01.069.0, P.O. Box 85090, 3508 AB, Utrecht, Netherlands aut de Jager Wilco Department of Paediatric Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, KC 01.069.0, P.O. Box 85090, 3508 AB, Utrecht, Netherlands aut Hoekstra Maarten Department of General Paediatrics, Wilhelmina Children's Hospital, Utrecht, Netherlands aut Knol Edward Department of Dermatology, University Medical Centre Utrecht, Utrecht, The Netherlands aut Prakken Berent Department of Paediatric Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, KC 01.069.0, P.O. Box 85090, 3508 AB, Utrecht, Netherlands aut Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 87-95 1355-8145 18:1<87 2013 18 12192 https://doi.org/10.1007/s12192-012-0361-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0361-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kapitein Berber Department of General Paediatrics, Wilhelmina Children's Hospital, Utrecht, Netherlands aut NATIONALLICENCE 700 1- Aalberse Joost Department of General Paediatrics, Wilhelmina Children's Hospital, Utrecht, Netherlands aut NATIONALLICENCE 700 1- Klein Mark Department of Paediatric Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, KC 01.069.0, P.O. Box 85090, 3508 AB, Utrecht, Netherlands aut NATIONALLICENCE 700 1- de Jager Wilco Department of Paediatric Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, KC 01.069.0, P.O. Box 85090, 3508 AB, Utrecht, Netherlands aut NATIONALLICENCE 700 1- Hoekstra Maarten Department of General Paediatrics, Wilhelmina Children's Hospital, Utrecht, Netherlands aut NATIONALLICENCE 700 1- Knol Edward Department of Dermatology, University Medical Centre Utrecht, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Prakken Berent Department of Paediatric Immunology, University Medical Centre Utrecht, Wilhelmina Children's Hospital, KC 01.069.0, P.O. Box 85090, 3508 AB, Utrecht, Netherlands aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 87-95 1355-8145 18:1<87 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer