naa a22 4500 510745849 CHVBK 20180411083045.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12192-012-0353-3 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0353-3 Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats [Elektronische Daten] [Felipe Saito, Débora Damasceno, Bruna Dallaqua, Iara Linhares, Marilza Rudge, Iracema De Mattos Paranhos Calderon, Steven Witkin] We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; non-treated non-diabetic rats (ND) served as controls. On day21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R = 0.5908, P = 0.0024; R = 0.4877, P = 0.0134, respectively) and the number of malformations per fetus (R = 0.6103, P = 0.0015; R = 0.4875, P = 0.0134, respectively). The anti-hsp60 IgG concentration was correlated with the number of malformations per fetus (R = 0.3887, P = 0.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R = 0.3999, P = 0.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes. Cell Stress Society International, 2012 Diabetes nationallicence Pregnancy nationallicence Malformation nationallicence Heat shock protein nationallicence Saito Felipe Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut Damasceno Débora Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut Dallaqua Bruna Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut Linhares Iara Department of Gynecology, University of Sao Paulo Medical School and Hospital das Clínicas, Sao Paulo, Brazil aut Rudge Marilza Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut De Mattos Paranhos Calderon Iracema Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut Witkin Steven Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 25-33 1355-8145 18:1<25 2013 18 12192 https://doi.org/10.1007/s12192-012-0353-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0353-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito Felipe Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Damasceno Débora Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Dallaqua Bruna Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Linhares Iara Department of Gynecology, University of Sao Paulo Medical School and Hospital das Clínicas, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Rudge Marilza Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- De Mattos Paranhos Calderon Iracema Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Witkin Steven Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/1(2013-01-01), 25-33 1355-8145 18:1<25 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer