naa a22 4500 510745865 CHVBK 20180411083045.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12192-013-0430-2 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0430-2 Lipocalin-2-mediated upregulation of various antioxidants and growth factors protects bone marrow-derived mesenchymal stem cells against unfavorable microenvironments [Elektronische Daten] [Raheleh Halabian, Hossein Tehrani, Ali Jahanian-Najafabadi, Mehryar Habibi Roudkenar] Despite many advantages of mesenchymal stem cells (MSCs) that make them suitable for cell therapy purposes, their therapeutic application has been limited due to their susceptibility to several stresses (e.g., nutrient-poor environment, oxidative stress, and hypoxic and masses of cytotoxic factors) to which they are exposed during their preparation and following transplantation. Hence, reinforcing MSCs against these stresses is a challenge for both basic and clinician scientists. Recently, much attention has been directed toward equipping MSCs with cytoprotective factors to strengthen them against unfavorable microenvironments. Here, we engineered MSCs with lipocalin 2 (Lcn2), a cytoprotective factor that is naturally induced following exposure of cells to stresses imposed by the microenvironment. Lcn2 overexpression not only did not interfere with the multidifferentiation capacity of the MSCs but also granted many protective properties to them. Lcn2 potentiated MSCs to withstand oxidative, hypoxia, and serum deprivation (SD) conditions via antagonizing their induced cytotoxicity and apoptosis. Adhesion rate of MSCs to coated culture plates was also enhanced by Lcn2 overexpression. In addition, Lcn2 induced antioxidants and upregulated some growth factors in MSCs. Our findings suggested a new strategy for prevention of graft cell death in MSC-based cell therapy. Cell Stress Society International, 2013 Mesenchymal stem cells nationallicence Lcn2 nationallicence Oxidative stress nationallicence Apoptosis nationallicence Antioxidants nationallicence Growth factors nationallicence Halabian Raheleh Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran aut Tehrani Hossein Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran aut Jahanian-Najafabadi Ali Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran aut Habibi Roudkenar Mehryar Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, P.O. Box 14665-1157, Tehran, Iran aut Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 785-800 1355-8145 18:6<785 2013 18 12192 https://doi.org/10.1007/s12192-013-0430-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0430-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Halabian Raheleh Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Tehrani Hossein Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran aut NATIONALLICENCE 700 1- Jahanian-Najafabadi Ali Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran aut NATIONALLICENCE 700 1- Habibi Roudkenar Mehryar Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, P.O. Box 14665-1157, Tehran, Iran aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 785-800 1355-8145 18:6<785 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer