naa a22 4500 510745881 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12192-013-0421-3 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0421-3 Association analysis of HSP70A1A haplotypes with heat tolerance in Chinese Holstein cattle [Elektronische Daten] [Qi Xiong, Jin Chai, Haiqian Xiong, Wengong Li, Tao Huang, Yang Liu, Xiaojun Suo, Nian Zhang, Xiaofeng Li, Siwen Jiang, Mingxin Chen] Single-nucleotide polymorphisms (SNPs) in the coding and untranslated regions of heat shock 70kDa protein 1A (HSP70A1A), an inducible molecular chaperone that is responsible for cellular protection against heat stress, have been reported as being associated with heat tolerance. A fragment of the HSP70A1A gene was amplified in Chinese Holstein cattle and eight novel mutations were found. We performed comprehensive linkage disequilibrium (LD) and haplotype analyses of the eight SNPs of the HSP70A1A gene and examined their involvement in heat resistance in 600 Chinese Holstein cattle. Our results revealed the presence of significant differences between individuals carrying haplotype 1 and those without haplotype 1 for most of the heat-tolerance traits. Haplotype 1 increased the risk of heat stress; however, association analysis of its combination with haplotype 2 showed the lowest rectal temperature and red blood cell K+ level, moderate respiratory rate, and the highest red blood cell NKA level, suggesting a heterozygote advantage in the penetration of the phenotype. Protein expression levels in white blood cells among haplotype combinations further confirmed the hypothesis that heterozygotes for haplotypes 1 and 2 are more sensitive to heat stress. We presume that these mutations may be useful in the future as molecular genetic markers to assist selection for heat tolerance in cattle. Cell Stress Society International, 2013 HSP70 nationallicence Heat stress nationallicence Haplotypes nationallicence Cattle nationallicence Association analysis nationallicence Xiong Qi Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut Chai Jin Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Xiong Haiqian Institute of Animal Husbandry and Veterinary, Huanggang Academy of Agricultural Sciences, 438000, Huanggang, China aut Li Wengong Institute of Animal Husbandry and Veterinary, Huanggang Academy of Agricultural Sciences, 438000, Huanggang, China aut Huang Tao Animal Husbandry and Veterinary Bureau of Hubei Province, 430064, Wuhan, China aut Liu Yang Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut Suo Xiaojun Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut Zhang Nian Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut Li Xiaofeng Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut Jiang Siwen Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Chen Mingxin Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 711-718 1355-8145 18:6<711 2013 18 12192 https://doi.org/10.1007/s12192-013-0421-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0421-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiong Qi Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut NATIONALLICENCE 700 1- Chai Jin Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Xiong Haiqian Institute of Animal Husbandry and Veterinary, Huanggang Academy of Agricultural Sciences, 438000, Huanggang, China aut NATIONALLICENCE 700 1- Li Wengong Institute of Animal Husbandry and Veterinary, Huanggang Academy of Agricultural Sciences, 438000, Huanggang, China aut NATIONALLICENCE 700 1- Huang Tao Animal Husbandry and Veterinary Bureau of Hubei Province, 430064, Wuhan, China aut NATIONALLICENCE 700 1- Liu Yang Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut NATIONALLICENCE 700 1- Suo Xiaojun Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Nian Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut NATIONALLICENCE 700 1- Li Xiaofeng Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut NATIONALLICENCE 700 1- Jiang Siwen Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Chen Mingxin Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, 430064, Wuhan, China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 711-718 1355-8145 18:6<711 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer