naa a22 4500 51074592X CHVBK 20180411083046.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12192-013-0423-1 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0423-1 Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX) [Elektronische Daten] [J. Kulbacka, M. Kotulska, N. Rembiałkowska, A. Choromańska, I. Kamińska, A. Garbiec, J. Rossowska, M. Daczewska, B. Jachimska, J. Saczko] Two porphyrins, CoTPPS and MnTMPyPCl5, were tested for their photodynamic activity and potential novel use in a therapy of human cancers. We investigated an effect of photodynamic reaction (PDR), electroporation (EP) and their combination (electro-photodynamic reaction [EP-PDR]) on human colon adenocarcinoma cell lines (LoVo and resistant to doxorubicin LoVoDX), human breast adenocarcinoma (wild type MCF-7/WT and resistant to doxorubicin MCF-7/DOX), and human melanoma (Me45). The efficiency of macromolecules transport was examined with cytofluorymetry by assessing the degree of propidium iodide (PI) penetration. Additionally, cellular ultrastructure after EP was evaluated. We determined cyto- and photo-cytotoxic effect on the cells viability (MTT assay) after standard PDR and PDR combined with EP. Intracellular distribution and mitochondrial colocalization of both porphyrins was also performed. The experiments proved that both complexes exhibit desirable photodynamic properties on LoVo LoVoDX cells, and EP effectively supports photodynamic method in this type of cancer. The application of EP provided shorter time of incubation (only 10min) and enhanced effect of applied therapy. The porphyrins did not affect the MCF-7 and Me45 cell lines. Cell Stress Society International, 2013 Electroporation nationallicence Photodynamic reaction nationallicence Porphyrins nationallicence Colon adenocarcinoma nationallicence PDR : Photodynamic reaction nationallicence PDT : Photodynamic therapy nationallicence EP : Electroporation nationallicence ECT : Electrochemotherapy nationallicence PI : Propidium iodide nationallicence MB : Myelin bodies nationallicence EDM : Electron dense material nationallicence Kulbacka J. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut Kotulska M. Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland aut Rembiałkowska N. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut Choromańska A. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut Kamińska I. Department of Pathomorphology and Clinical Cytology, Medical University, Borowska 213 Street, 50-556, Wroclaw, Poland aut Garbiec A. Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21, 50-335, Wroclaw, Poland aut Rossowska J. Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigla 12 Street, 53-114, Wroclaw, Poland aut Daczewska M. Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21, 50-335, Wroclaw, Poland aut Jachimska B. J. Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239, Krakow, Poland aut Saczko J. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 719-731 1355-8145 18:6<719 2013 18 12192 https://doi.org/10.1007/s12192-013-0423-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0423-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kulbacka J. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut NATIONALLICENCE 700 1- Kotulska M. Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland aut NATIONALLICENCE 700 1- Rembiałkowska N. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut NATIONALLICENCE 700 1- Choromańska A. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut NATIONALLICENCE 700 1- Kamińska I. Department of Pathomorphology and Clinical Cytology, Medical University, Borowska 213 Street, 50-556, Wroclaw, Poland aut NATIONALLICENCE 700 1- Garbiec A. Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21, 50-335, Wroclaw, Poland aut NATIONALLICENCE 700 1- Rossowska J. Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigla 12 Street, 53-114, Wroclaw, Poland aut NATIONALLICENCE 700 1- Daczewska M. Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21, 50-335, Wroclaw, Poland aut NATIONALLICENCE 700 1- Jachimska B. J. Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239, Krakow, Poland aut NATIONALLICENCE 700 1- Saczko J. Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 719-731 1355-8145 18:6<719 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer