naa a22 4500 510745938 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12192-013-0429-8 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0429-8 The role of heat shock proteins in inflammatory injury induced by cold stress in chicken hearts [Elektronische Daten] [Fu-Qing Zhao, Zi-Wei Zhang, Chao Wang, Bo Zhang, Hai-Dong Yao, Shu Li, Shi-Wen Xu] The aim of this study was to investigate the effects of cold stress on the expression levels of heat shock proteins (Hsps90, 70, 60, 40, and 27) and inflammatory factors (iNOS, COX-2, NF-κB, TNF-α, and PTGEs) and oxidative indexes in hearts of chickens. Two hundred forty 15-day-old male chickens were randomly divided into 12 groups and kept at the temperature of 12 ± 1°C for acute and chronic cold stress. There were one control group and five treatment groups for acute cold stress, three control groups, and three treatment groups for chronic cold stress. After cold stress, malondialdehyde level increased in chicken heart; the activity of superoxide dismutase and glutathione peroxidase in the heart first increased and then decreased. The inflammatory factors mRNA levels were increased in cold stress groups relative to control groups. The histopathological analysis showed that heart tissues were seriously injured in the cold stress group. Additionally, the mRNA levels of Hsps (70, 60, 40, and 27) increased significantly (P < 0.05) in the cold stress groups relative to the corresponding control group. Meanwhile, the mRNA level and protein expression of Hsp90 decreased significantly (P < 0.05) in the stress group, and showed a gradually decreasing tendency. These results suggested that the levels of inflammatory factors and Hsps expression levels in heart tissues can be influenced by cold stress. Hsps commonly played an important role in the protection of the heart after cold stress. Cell Stress Society International, 2013 Cold stress nationallicence Heat shock proteins nationallicence Inflammatory injury nationallicence Chicken heart nationallicence Zhao Fu-Qing Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Zhang Zi-Wei Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Wang Chao Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Zhang Bo Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Yao Hai-Dong Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Li Shu Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Xu Shi-Wen Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 773-783 1355-8145 18:6<773 2013 18 12192 https://doi.org/10.1007/s12192-013-0429-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0429-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Fu-Qing Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zi-Wei Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Wang Chao Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Bo Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Yao Hai-Dong Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Li Shu Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Xu Shi-Wen Department of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 773-783 1355-8145 18:6<773 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer