naa a22 4500 510745970 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12192-013-0422-2 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0422-2 Capsaicin as an inducer of damage-associated molecular patterns (DAMPs) of immunogenic cell death (ICD) in human bladder cancer cells [Elektronische Daten] [Donatella D'Eliseo, Laura Manzi, Francesca Velotti] Few conventional cytotoxic anticancer therapeutics induce immunogenic cell death (ICD). This means that they induce tumor cells to undergo apoptosis while eliciting the emission of a spatiotemporal-defined combination of damage-associated molecular patterns (DAMPs) decoded by the immune system to activate antitumor immunity effective for long-term therapeutic success. The neurotoxin capsaicin (CPS) can induce both cancer cell apoptosis and immune-mediated tumor regression. In the present study, we investigated whether CPS is capable of eliciting the emission of ICD hallmarks in human bladder cancer cell lines undergoing apoptosis. We demonstrated that CPS induces pre- and early apoptotic cell surface exposure of calreticulin (CRT), HSP90, and HSP70 as well as ATP release. Moreover, CRT exposure was prevented by inhibition of endoplasmic reticulum-Golgi traffic by brefeldin A. Furthermore, high-mobility group box 1, HSP90, and HSP70 were passively released at late apoptotic stages. We provide the first evidence that CPS is an inducer of ICD hallmarks, suggesting CPS as a novel potential immunogenic cytotoxic agent. Cell Stress Society International, 2013 Capsaicin nationallicence Immunogenic cell death nationallicence Calreticulin nationallicence Heat shock proteins nationallicence Adenosine triphosphate nationallicence High-mobility group box 1 nationallicence D'Eliseo Donatella Department of Ecological and Biological Sciences (DEB), University of La Tuscia, Largo dell'Università, Blocco C, 01100, Viterbo, Italy aut Manzi Laura Department of Ecological and Biological Sciences (DEB), University of La Tuscia, Largo dell'Università, Blocco C, 01100, Viterbo, Italy aut Velotti Francesca Department of Ecological and Biological Sciences (DEB), University of La Tuscia, Largo dell'Università, Blocco C, 01100, Viterbo, Italy aut Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 801-808 1355-8145 18:6<801 2013 18 12192 https://doi.org/10.1007/s12192-013-0422-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0422-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- D'Eliseo Donatella Department of Ecological and Biological Sciences (DEB), University of La Tuscia, Largo dell'Università, Blocco C, 01100, Viterbo, Italy aut NATIONALLICENCE 700 1- Manzi Laura Department of Ecological and Biological Sciences (DEB), University of La Tuscia, Largo dell'Università, Blocco C, 01100, Viterbo, Italy aut NATIONALLICENCE 700 1- Velotti Francesca Department of Ecological and Biological Sciences (DEB), University of La Tuscia, Largo dell'Università, Blocco C, 01100, Viterbo, Italy aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/6(2013-11-01), 801-808 1355-8145 18:6<801 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer