naa a22 4500 510745989 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0379-6 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0379-6 Heat shock protein 27 is increased in cyanotic tetralogy of Fallot myocardium and is associated with improved cardiac output and contraction [Elektronische Daten] [Susan Walker, Mark Danton, Edward Peng, Fiona Lyall] Tetralogy of Fallot (TOF) is a congenital heart condition in which the right ventricle is exposed to cyanosis and pressure overload. Patients have an increased risk of right ventricle dysfunction following corrective surgery. Whether the cyanotic myocardium is less tolerant of injury compared to non-cyanotic is unclear. Heat shock proteins (HSPs) protect against cellular stresses. The aim of this study was to examine HSP 27 expression in the right ventricle resected from TOF patients and determine its relationship with right ventricle function and clinical outcome. Ten cyanotic and ten non-cyanotic patients were studied. Western blotting was used to quantify HSP 27 in resected myocardium at (1) baseline (first 15min of aortic cross clamp and closest representation of pre-operative status) and (2) after 15min during ischemia until surgery was complete. The cyanotic group had significantly increased haematocrit, lower O2 saturation, thicker interventricular septal wall thickness and released more troponin-I on post-operative day 1 (p < 0.05). HSP 27 expression was significantly increased in the <15min cyanotic compared to the <15min non-cyanotic group (p = 0.03). In the cyanotic group, baseline HSP 27 expression also significantly correlated with oxygen extraction ratio (p = 0.028), post-operative basal septal velocity (p = 0.036) and mixed venous oxygen saturation (p = 0.02), markers of improved cardiac output/contraction. Increased HSP 27 expression and associated improved right ventricle function and systemic perfusion supports a cardio-protective effect of HSP 27 in cyanotic TOF. Cell Stress Society International, 2012 Tetralogy of Fallot nationallicence Heat shock protein 27 nationallicence Right ventricle nationallicence Myocardium nationallicence Cyanotic nationallicence Non-cyanotic nationallicence Walker Susan Institute of Medical Genetics, G38SJ, Yorkhill, Glasgow, UK aut Danton Mark Department of Cardiac Surgery, Royal Hospital for Sick Children, G38SJ, Glasgow, UK aut Peng Edward Department of Cardiac Surgery, Royal Hospital for Sick Children, G38SJ, Glasgow, UK aut Lyall Fiona Institute of Medical Genetics, G38SJ, Yorkhill, Glasgow, UK aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 269-277 1355-8145 18:3<269 2013 18 12192 https://doi.org/10.1007/s12192-012-0379-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0379-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walker Susan Institute of Medical Genetics, G38SJ, Yorkhill, Glasgow, UK aut NATIONALLICENCE 700 1- Danton Mark Department of Cardiac Surgery, Royal Hospital for Sick Children, G38SJ, Glasgow, UK aut NATIONALLICENCE 700 1- Peng Edward Department of Cardiac Surgery, Royal Hospital for Sick Children, G38SJ, Glasgow, UK aut NATIONALLICENCE 700 1- Lyall Fiona Institute of Medical Genetics, G38SJ, Yorkhill, Glasgow, UK aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 269-277 1355-8145 18:3<269 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer