naa a22 4500 510746004 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0389-4 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0389-4 PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line [Elektronische Daten] [Bo-Shi Wang, Yang Yang, Hai Yang, Yi-Zhen Liu, Jia-Jie Hao, Yu Zhang, Zhi-Zhou Shi, Xue-Mei Jia, Qi-Min Zhan, Ming-Rong Wang] Using a glutathione S-transferase pull-down liquid chromatography-coupled tandem mass spectrometry approach and immunoprecipitation/immunoblot analysis, we found that heat shock cognate protein 70 (Hsc70) was involved in the complex formed by atypical protein kinase Cι (PKCι) and LC3 in the esophageal cancer cell line KYSE30. Further study indicated that Hsc70 was targeted by autophagic degradation, and knockdown of PKCι down-regulated Hsc70 by promoting autophagy. PKCι knockdown sensitized cells to oxidative stress-induced apoptosis, whereas forced PKCι expression counteracted the oxidative stress-induced apoptosis via Hsc70. Cell Stress Society International, 2012 PKCι nationallicence Hsc70 nationallicence Oxidative stress nationallicence Autophagic degradation nationallicence LC : Liquid chromatography nationallicence MS : Mass spectrometry nationallicence PKCι : Atypical protein kinase Cι nationallicence Hsc70 : Heat shock cognate protein 70 nationallicence Wang Bo-Shi State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Yang Yang State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Yang Hai State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Liu Yi-Zhen State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Hao Jia-Jie State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Zhang Yu State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Shi Zhi-Zhou State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Jia Xue-Mei Department of Histology and Embryology, Anhui Medical University, Hefei, China aut Zhan Qi-Min State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Wang Ming-Rong State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 359-366 1355-8145 18:3<359 2013 18 12192 https://doi.org/10.1007/s12192-012-0389-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0389-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Bo-Shi State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Yang Yang State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Yang Hai State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Liu Yi-Zhen State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Hao Jia-Jie State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Zhang Yu State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Shi Zhi-Zhou State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Jia Xue-Mei Department of Histology and Embryology, Anhui Medical University, Hefei, China aut NATIONALLICENCE 700 1- Zhan Qi-Min State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 700 1- Wang Ming-Rong State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 359-366 1355-8145 18:3<359 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer