naa a22 4500 510746012 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0388-5 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0388-5 Circulating anti-Hsp70 levels in nascent metabolic syndrome: the Casale Monferrato Study [Elektronische Daten] [Gabriella Gruden, Federica Barutta, Silvia Pinach, Bartolomeo Lorenzati, Paolo Cavallo-Perin, Sara Giunti, Graziella Bruno] The metabolic syndrome (MetS) confers an increased risk of both type 2 diabetes and cardiovascular diseases (CVD). Heat shock protein 70 (Hsp70), an intracellular polypeptide, can be exposed on the plasma membrane and/or released into the circulation, eliciting both native and immune responses that may contribute to vascular damage. Our aim was to assess if serum anti-Hsp70 antibody levels were cross-sectionally associated with uncomplicated MetS. A cross-sectional case-control study from the nondiabetic cohort of the Casale Monferrato Study was performed. Subjects with established CVD and/or abnormal renal function were excluded. Case subjects (n = 180) were defined as those fulfilling the criteria for the diagnosis of MetS. Control subjects (n = 136) were completely free of any component of the MetS. Serum anti-Hsp70 levels were measured by immunoenzymatic assay. We found that anti-Hsp70 antibody levels were significantly higher in cases than in control subjects [122.6 (89.5-155.6) vs 107.1 (77.3-152.4) μg/ml, p = 0.04], even after age and sex adjustment. In logistic regression analysis, higher levels of log-anti-Hsp70 conferred greater odds ratio (OR) for MetS, independently of age and sex. There was a statistically significant trend of ORs across quartiles of anti-Hsp70 and values greater than 108.0μg/ml conferred a 77% increased OR of MetS as compared with values in the lower quartiles. The strength of the association slightly decreased after further adjustment for apolipoprotein B, smoking, and albumin excretion rate. In conclusion, our results show that serum anti-Hsp70 antibody levels are independently associated with nascent MetS. Cell Stress Society International, 2012 Metabolic syndrome nationallicence Heat shock proteins nationallicence Anti-heat shock protein 70 antibodies nationallicence Cardiovascular disease nationallicence Gruden Gabriella Department of Medical Sciences, University of Turin, Turin, Italy aut Barutta Federica Department of Medical Sciences, University of Turin, Turin, Italy aut Pinach Silvia Department of Medical Sciences, University of Turin, Turin, Italy aut Lorenzati Bartolomeo Department of Medical Sciences, University of Turin, Turin, Italy aut Cavallo-Perin Paolo Department of Medical Sciences, University of Turin, Turin, Italy aut Giunti Sara Department of Medical Sciences, University of Turin, Turin, Italy aut Bruno Graziella Department of Medical Sciences, University of Turin, Turin, Italy aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 353-357 1355-8145 18:3<353 2013 18 12192 https://doi.org/10.1007/s12192-012-0388-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0388-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gruden Gabriella Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Barutta Federica Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Pinach Silvia Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Lorenzati Bartolomeo Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Cavallo-Perin Paolo Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Giunti Sara Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Bruno Graziella Department of Medical Sciences, University of Turin, Turin, Italy aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 353-357 1355-8145 18:3<353 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer