naa a22 4500 510746039 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0386-7 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0386-7 Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock [Elektronische Daten] [Zhe Jing, Rajendra Gangalum, Josh Lee, Dennis Mock, Suraj Bhat] Epithelial cells and fibroblasts both express heat shock transcription factors, HSF1 and HSF4, yet they respond to heat shock differentially. For example, while HSP70 is induced in both cell types, the small heat shock protein, αB-crystallin gene (CRYAB) that contains a canonical heat shock promoter, is only induced in fibroblasts. A canonical heat shock promoter contains three or more inverted repeats of the pentanucleotide 5′-nGAAn-3′ that make the heat shock element. It is known that, in vitro, promoter architecture (the order and spacing of these repeats) impacts the interaction of various heat shock transcription factors (HSFs) with the heat shock promoter, but in vivo relevance of these binding preferences so far as the expression is concerned is poorly understood. In this report, we first establish cell-type-dependent differential expression of CRYAB in four established cell lines and then working with adult human retinal pigment epithelial cells and NIH3T3 fibroblasts and employing chromatin immunoprecipitation, attempt to relate expression to promoter occupancy by HSF1 and HSF4. We show that HSF4 occupies only CRYAB and not HSP70 promoter in epithelial cells, while HSF1 occupies only HSP70 promoter in both cell types, and cryab promoter, only in heat shocked fibroblasts; HSF4, on the other hand, is never seen on these two promoters in NIH3T3 fibroblasts. This comparative analysis with CRYAB and HSP70 demonstrates that differential heat shock response is controlled by cell-type-dependent access of HSFs (HSF1 and HSF4) to specific promoters, independent of the promoter architecture. Cell Stress Society International, 2012 Heat shock promoter nationallicence HSF1 nationallicence HSF4 nationallicence Cell-type specific nationallicence HSP70 nationallicence αB-crystallin nationallicence Jing Zhe Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut Gangalum Rajendra Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut Lee Josh Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut Mock Dennis Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut Bhat Suraj Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 377-387 1355-8145 18:3<377 2013 18 12192 https://doi.org/10.1007/s12192-012-0386-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0386-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jing Zhe Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Gangalum Rajendra Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Lee Josh Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Mock Dennis Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Bhat Suraj Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Brain Research Institute and Molecular Biology Institute, University of California, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 377-387 1355-8145 18:3<377 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer