naa a22 4500 510746047 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0391-x doi (NATIONALLICENCE)springer-10.1007/s12192-012-0391-x Hsp70 chaperone-based gel composition as a novel immunotherapeutic anti-tumor tool [Elektronische Daten] [Sergey Abkin, Katerina Pankratova, Elena Komarova, Irina Guzhova, Boris Margulis] The recent advances in designing Hsp70-based anti-cancer vaccines and the ability of the chaperone to penetrate inside a living cell prompted us to develop a non-invasive method for the treatment of surface tumors. We designed hydrogel-containing gel-forming substances and human recombinant Hsp70 and applied them on the surface of a 7-day-old B16F10 melanoma tumor. According to the results of histochemistry, Hsp70 diffused through skin layer inside the B16 tumor, and this transport was proved by biochemical data. The application of Hsp70 gel reduced the rate of tumor growth by 64% and prolonged the life of animals by 46%. Increased survival was correlated with the enhancement of B16-specific cytotoxicity and up-regulation of gamma-interferon production. Taken together, the data confirm the anti-tumor effect of pure recombinant Hsp70 delivered intratumorally and demonstrate the relevance of a novel non-invasive technology of Hsp70-based therapy. Cell Stress Society International, 2012 Chaperone Hsp70 nationallicence Melanoma nationallicence Tumor nationallicence Gamma-interferon nationallicence CTL nationallicence Abkin Sergey Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut Pankratova Katerina Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut Komarova Elena Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut Guzhova Irina Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut Margulis Boris Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 391-396 1355-8145 18:3<391 2013 18 12192 https://doi.org/10.1007/s12192-012-0391-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0391-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abkin Sergey Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Pankratova Katerina Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Komarova Elena Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Guzhova Irina Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Margulis Boris Laboratory of Cell Protection Mechanisms, Institute of Cytology of Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 391-396 1355-8145 18:3<391 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer