naa a22 4500 510746063 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0383-x doi (NATIONALLICENCE)springer-10.1007/s12192-012-0383-x A modified UPR stress sensing system reveals a novel tissue distribution of IRE1/XBP1 activity during normal Drosophila development [Elektronische Daten] [Michio Sone, Xiaomei Zeng, Joseph Larese, Hyung Ryoo] Eukaryotic cells respond to stress caused by the accumulation of unfolded/misfolded proteins in the endoplasmic reticulum by activating the intracellular signaling pathways referred to as the unfolded protein response (UPR). In metazoans, UPR consists of three parallel branches, each characterized by its stress sensor protein, IRE1, ATF6, and PERK, respectively. In Drosophila, IRE1/XBP1 pathway is considered to function as a major branch of UPR; however, its physiological roles during the normal development and homeostasis remain poorly understood. To visualize IRE1/XBP1 activity in fly tissues under normal physiological conditions, we modified previously reported XBP1 stress sensing systems (Souid et al., Dev Genes Evol 217: 159-167, 2007; Ryoo et al., EMBO J 26: 242-252, 2007), based on the recent reports regarding the unconventional splicing of XBP1/HAC1 mRNA (Aragon et al., Nature 457: 736-740, 2009; Yanagitani et al., Mol Cell 34: 191-200, 2009; Science 331: 586-589, 2011). The improved XBP1 stress sensing system allowed us to detect new IRE1/XBP1 activities in the brain, gut, Malpighian tubules, and trachea of third instar larvae and in the adult male reproductive organ. Specifically, in the larval brain, IRE1/XBP1 activity was detected exclusively in glia, although previous reports have largely focused on IRE1/XBP1 activity in neurons. Unexpected glial IRE1/XBP1 activity may provide us with novel insights into the brain homeostasis regulated by the UPR. Cell Stress Society International, 2012 UPR nationallicence ER stress nationallicence XBP1 nationallicence Drosophila nationallicence Glia nationallicence Sone Michio Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut Zeng Xiaomei Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut Larese Joseph Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut Ryoo Hyung Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 307-319 1355-8145 18:3<307 2013 18 12192 https://doi.org/10.1007/s12192-012-0383-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0383-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sone Michio Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut NATIONALLICENCE 700 1- Zeng Xiaomei Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut NATIONALLICENCE 700 1- Larese Joseph Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut NATIONALLICENCE 700 1- Ryoo Hyung Department of Cell Biology, New York University School of Medicine, 560 First Avenue, 10016, New York, NY, USA aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 307-319 1355-8145 18:3<307 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer