naa a22 4500 510746101 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12192-012-0390-y doi (NATIONALLICENCE)springer-10.1007/s12192-012-0390-y Improvements in the primary culture of neonate rat myocardial cells by study of the mechanism of endoplasmic reticulum stress [Elektronische Daten] [Qiqi Wang, Jianmei Jin, Zhidong Guo, Fuxu Chen, Yuangang Qiu, Jianhua Zhu, Yunpeng Shang] We previously found that endoplasmic reticulum stress (ERS) might be exhibited in the conventional protocol of the primary culture of neonate rat myocardial cells (NRMCs) and that the high glucose concentration (25mmol/L) in the culture medium might be the cause. Here, we investigated if the high concentration of glucose might influence ERS in myocardial cells during culture. GRP78 expression (ERS marker) was similar in groups with tunicamycin (TM) and without TM in high glucose cultured cells (p > 0.01). Different glucose concentrations elicited different GRP78 expressions according to analyses of protein and RNA levels, which showed ERS in H/H groups. Finally, we found that GRP78 expression was higher in TM groups compared with M/M groups (p < 0.01). The conventional high-glucose culture media during primary culture of NRMCs induced ERS. We propose that medium-glucose culture media should be used and describe an improved protocol for the primary culture of NRMCs. Cell Stress Society International, 2013 Endoplasmic reticulum stress nationallicence Primary culture of neonate rat myocardial cells nationallicence Culture technique nationallicence Wang Qiqi Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut Jin Jianmei Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut Guo Zhidong Department of Emergency, First People's Hospital of Hangzhou, Hangzhou, Zhejiang Province, People's Republic of China aut Chen Fuxu Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut Qiu Yuangang Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut Zhu Jianhua Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut Shang Yunpeng Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 367-375 1355-8145 18:3<367 2013 18 12192 https://doi.org/10.1007/s12192-012-0390-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0390-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Qiqi Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 700 1- Jin Jianmei Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 700 1- Guo Zhidong Department of Emergency, First People's Hospital of Hangzhou, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 700 1- Chen Fuxu Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 700 1- Qiu Yuangang Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Jianhua Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 700 1- Shang Yunpeng Department of Cardiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang Province, People's Republic of China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/3(2013-05-01), 367-375 1355-8145 18:3<367 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer