naa a22 4500 51074611X CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-012-0398-3 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0398-3 Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm [Elektronische Daten] [Myung-Hee Kim, Yutaka Sonoda, Kentaro Sasaki, Hironori Kaminaka, Ryozo Imai] Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares an RNA chaperone function with E. coli cold shock proteins and regulates freezing tolerance during cold acclimation. Here, we screened for AtCSP3-interacting proteins using a yeast two-hybrid system and 38 candidate interactors were identified. Sixteen of these were further confirmed in planta interaction between AtCSP3 by a bi-molecular fluorescence complementation assay. We found that AtCSP3 interacts with CONSTANS-LIKE protein 15 and nuclear poly(A)-binding proteins in nuclear speckles. Three 60S ribosomal proteins (RPL26A, RPL40A/UBQ2, and RPL36aB) and the Gar1 RNA-binding protein interacted with AtCSP3 in the nucleolus and nucleoplasm, suggesting that AtCSP3 functions in ribosome biogenesis. Interactions with LOS2/enolase and glycine-rich RNA-binding protein 7 that are cold inducible, and an mRNA decapping protein 5 (DCP5) were observed in the cytoplasm. These data suggest that AtCSP3 participates in multiple complexes that reside in nuclear and cytoplasmic compartments and possibly regulates RNA processing and functioning. Cell Stress Society International, 2013 Cold shock protein nationallicence RNA chaperone nationallicence Nucleus nationallicence Cytoplasm nationallicence Arabidopsis thaliana nationallicence mRNA processing nationallicence BiFC : Bi-molecular fluorescence complementation nationallicence COL : CONSTANS-LIKE protein nationallicence CBF : C-repeat-binding factor nationallicence CSD : Cold shock domain nationallicence CSPs : Cold shock domain protein nationallicence DCP : Decapping protein nationallicence Gar : Glycine-arginine rich domain nationallicence GFP : Green fluorescent protein nationallicence GRP : Glycine-rich RNA-binding protein nationallicence LOS : Low expression of osmotically responsive gene nationallicence PABN : Nuclear type of poly(A)-binding protein nationallicence RPL : Ribosomal protein nationallicence YFP : Yellow fluorescent protein nationallicence Kim Myung-Hee Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut Sonoda Yutaka Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut Sasaki Kentaro Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut Kaminaka Hironori Laboratory of Plant Molecular Biology, Faculty of Agriculture, Tottori University, Tottori, Japan aut Imai Ryozo Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 517-525 1355-8145 18:4<517 2013 18 12192 https://doi.org/10.1007/s12192-012-0398-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0398-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Myung-Hee Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut NATIONALLICENCE 700 1- Sonoda Yutaka Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut NATIONALLICENCE 700 1- Sasaki Kentaro Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut NATIONALLICENCE 700 1- Kaminaka Hironori Laboratory of Plant Molecular Biology, Faculty of Agriculture, Tottori University, Tottori, Japan aut NATIONALLICENCE 700 1- Imai Ryozo Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, 062-8555, Sapporo, Japan aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 517-525 1355-8145 18:4<517 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer