naa a22 4500 510746128 CHVBK 20180411083046.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-012-0392-9 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0392-9 Increased CCT-eta expression is a marker of latent and active disease and a modulator of fibroblast contractility in Dupuytren's contracture [Elektronische Daten] [Latha Satish, David O'Gorman, Sandra Johnson, Christina Raykha, Bing Gan, James Wang, Sandeep Kathju] Dupuytren's contracture (DC) is a fibroproliferative disorder of unknown etiology characterized by a scar-like contracture that develops in the palm and/or digits. We have previously reported that the eta subunit of the chaperonin containing T-complex polypeptide (CCT-eta) is increased in fibrotic wound healing, and is essential for the accumulation of α-smooth muscle actin (α-SMA) in fibroblasts. The purpose of this study was to determine if CCT-eta is similarly implicated in the aberrant fibrosis seen in DC and to investigate the role of CCT-eta in the behavior of myo/fibroblasts in DC. Fibroblasts were obtained from DC-affected palmar fascia, from adjacent phenotypically normal palmar fascia in the same DC patients (PF), and from non-DC palmar fascial tissues in patients undergoing carpal tunnel (CT) release. Inherent contractility in these three populations was examined using fibroblast-populated collagen lattices (FPCLs) and by cell traction force microscopy. Expression of CCT-eta and α-SMA protein was determined by Western blot. The effect of CCT-eta inhibition on the contractility of DC cells was determined by deploying an siRNA versus CCT-eta. DC cells were significantly more contractile than both matching palmar fascial (PF) cells and CT cells in both assays, with PF cells demonstrating an intermediate contractility in the FPCL assay. Whereas α-SMA protein was significantly increased only in DC cells compared to PF and CT cells, CCT-eta protein was significantly increased in both PF and DC cells compared to CT cells. siRNA-mediated depletion of CCT-eta inhibited the accumulation of both CCT-eta and α-SMA protein in DC cells, and also significantly decreased the contractility of treated DC cells. These observations suggest that increased expression of CCT-eta appears to be a marker for latent and active disease in these patients and to be essential for the increased contractility exhibited by these fibroblasts. Cell Stress Society International, 2013 Dupuytren's contracture nationallicence Palmar fascia nationallicence Fibroblast nationallicence Contractility nationallicence Traction force nationallicence CCT-eta nationallicence α-Smooth muscle actin nationallicence Satish Latha Department of Plastic Surgery, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut O'Gorman David Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, St. Joseph's Hospital, London, ON, Canada aut Johnson Sandra Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, 15212, Pittsburgh, PA, USA aut Raykha Christina Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, St. Joseph's Hospital, London, ON, Canada aut Gan Bing Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, St. Joseph's Hospital, London, ON, Canada aut Wang James The MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Kathju Sandeep Department of Plastic Surgery, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 397-404 1355-8145 18:4<397 2013 18 12192 https://doi.org/10.1007/s12192-012-0392-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0392-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Satish Latha Department of Plastic Surgery, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- O'Gorman David Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, St. Joseph's Hospital, London, ON, Canada aut NATIONALLICENCE 700 1- Johnson Sandra Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, 15212, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Raykha Christina Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, St. Joseph's Hospital, London, ON, Canada aut NATIONALLICENCE 700 1- Gan Bing Cell and Molecular Biology Laboratory of the Hand and Upper Limb Centre, St. Joseph's Hospital, London, ON, Canada aut NATIONALLICENCE 700 1- Wang James The MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Kathju Sandeep Department of Plastic Surgery, University of Pittsburgh, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 397-404 1355-8145 18:4<397 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer