naa a22 4500 510746179 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-013-0402-6 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0402-6 Expression of Hsp70 in the mud crab, Scylla paramamosain in response to bacterial, osmotic, and thermal stress [Elektronische Daten] [Ya'nan Yang, Haihui Ye, Huiyang Huang, Shaojing Li, Xueliang Liu, Xianglan Zeng, Jie Gong] Hsp70 is involved in immune responses against infectious pathogens, thermal, and osmotic stress. To understand the immune defense mechanisms of the mud crab Scylla paramamosain, genomic DNA, transcript level and antimicrobial activities of Hsp70 were analyzed. Genomic DNA sequence analysis revealed one intron in this gene. Furthermore, six SNPs were detected by direct sequencing from 30 samples in this study. Hsp70 mRNA was expressed in almost all tissues examined. By using the quantitative real-time PCR, the expression level of Hsp70 in hemocytes showed a clear time-dependent expression pattern during the 96h after stimulated by Vibrio alginolyticus. Then, recombinant Hsp70 was obtained by using the bacterial expression system, but no obvious antimicrobial activity has been found for the protein in the antimicrobial tests. After osmotic stress, the expression of Hsp70 in hemocytes showed this gene was induced by the high salinity (30 ‰) for at least 96h. Hsp70 mRNA expression in hemocytes was analyzed after thermal stress at 6h, the highest and the lowest expression level of Hsp70 was observed at 36 and 15°C, respectively. These results indicated that Hsp70 was inducible by bacterial, osmotic, and thermal stress, and therefore plays an important role, different from antibacterial peptide, in innate immune responses of S. paramamosain. Cell Stress Society International, 2013 Scylla paramamosain nationallicence Heat shock protein70 (Hsp70) nationallicence mRNA expression nationallicence SNP nationallicence Different stress nationallicence Recombinant Hsp70 nationallicence Yang Ya'nan College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Ye Haihui College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Huang Huiyang College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Li Shaojing College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Liu Xueliang College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Zeng Xianglan College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Gong Jie College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 475-482 1355-8145 18:4<475 2013 18 12192 https://doi.org/10.1007/s12192-013-0402-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0402-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Ya'nan College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Ye Haihui College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Huang Huiyang College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Li Shaojing College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Liu Xueliang College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Zeng Xianglan College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Gong Jie College of Ocean and Earth Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 475-482 1355-8145 18:4<475 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer