naa a22 4500 510746187 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-012-0397-4 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0397-4 Mycobacterial and mouse HSP70 have immuno-modulatory effects on dendritic cells [Elektronische Daten] [R. Spiering, R. van der Zee, J. Wagenaar, W. van Eden, F. Broere] Previously, it has been shown that heat shock protein 70 (HSP70) can prevent inflammatory damage in experimental autoimmune disease models. Various possible underlying working mechanisms have been proposed. One possibility is that HSP70 induces a tolerogenic phenotype in dendritic cells (DCs) as a result of the direct interaction of the antigen with the DC. Tolerogenic DCs can induce antigen-specific regulatory T cells and dampen pathogenic T cell responses. We show that treatment of murine DCs with either mycobacterial (Mt) or mouse HSP70 and pulsed with the disease-inducing antigen induced suppression of proteoglycan-induced arthritis (PGIA), although mouse HSP70-treated DCs could ameliorate PGIA to a greater extent. In addition, while murine DCs treated with Mt- or mouse HSP70 had no significantly altered phenotype as compared to untreated DCs, HSP70-treated DCs pulsed with pOVA (ovalbumin peptide 323-339) induced a significantly increased production of IL-10 in pOVA-specific T cells. IL-10-producing T cells were earlier shown to be involved in Mt HSP70-induced suppression of PGIA. In conclusion, this study indicates that Mt- and mouse HSP70-treated BMDC can suppress PGIA via an IL-10-producing T cell-dependent manner. Cell Stress Society International, 2012 Dendritic cell nationallicence HSP70 nationallicence Arthritis nationallicence Mouse/murine nationallicence Tolerance nationallicence Spiering R. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut van der Zee R. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut Wagenaar J. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut van Eden W. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut Broere F. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 439-446 1355-8145 18:4<439 2013 18 12192 https://doi.org/10.1007/s12192-012-0397-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0397-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Spiering R. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van der Zee R. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Wagenaar J. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van Eden W. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Broere F. Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 439-446 1355-8145 18:4<439 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer