naa a22 4500 510746209 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-013-0404-4 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0404-4 Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis [Elektronische Daten] [Xue Leng, Xinxing Wang, Wei Pang, Rui Zhan, Zhiqing Zhang, Liqun Wang, Xiujie Gao, Lingjia Qian] Although previous studies have shown that autoantigens such as Hsps have been implicated by induction of an autoimmune process in the development of atherosclerosis, the exact role of anti-Hsp70 antibody in atherosclerosis is unknown. In the present study, the levels of anti-Hsp70 autoantibodies and oxidized low density lipoprotein (OxLDL) were all significantly increased, and they were strongly correlated in an atherosclerosis model. After the endothelial cells were incubated with 20μg/mL OxLDL for 12h at 37°C and followed by 90min recovery, Hsp70 positive staining of OxLDL-treated endothelial cells was observed on the cell surface in immunostaining and flow cytometric analysis. This membrane Hsp70 was not from culture supernatant Hsp70 and binding of extracellular Hsp70 but was defined as endothelial surface membrane Hsp70. Furthermore, only in the OxLDL-treated group, but not in the untreated group, 51Cr-labeled endothelial cells were lysed by anti-Hsp70 antibody (BD091, IgAS) in the presence of either complement or peripheral blood mononuclear cells. Control antibodies, including IgNor, mAb to Hsp70 (SPA-810), and mAbs to Factor VIII, α-actin, and CD3 showed no cytotoxic effects. In conclusion, anti-Hsp70 antibodies could be reacting with the endothelial surface membrane Hsp70 induced by OxLDL and were able to mediate endothelial cytotoxicity. There is a possibility that a humoral immune reaction to endothelial surface membrane Hsp70 may play an important role in the pathogenesis of atherosclerosis. Cell Stress Society International, 2013 Endothelial cell nationallicence Surface membrane Hsp70 nationallicence Anti-Hsp70 antibody nationallicence OxLDL nationallicence Atherosclerosis nationallicence Hsps : Heat shock proteins nationallicence OxLDL : Oxidized low density lipoprotein nationallicence AS : Atherosclerosis nationallicence IgAS : Atherosclerotic rat plasma Hsp70 autoantibodies nationallicence IgNor : Control rat plasma Hsp70 autoantibodies nationallicence mAb : Monoclonal antibody nationallicence BD091 : Monoclonal antibody to Hsp70 (which recognized Hsp70 C-terminal) nationallicence SPA-810 : Monoclonal antibody to Hsp70 (binding site, aa437-504) nationallicence PBS : Phosphate-buffered saline nationallicence HRP : Horseradish peroxidase nationallicence TMB : Tetramethyl benzidine nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence Leng Xue Institute of Medical Equipment, 300161, Tianjin, People's Republic of China aut Wang Xinxing Institute of Basic Medical Sciences, 100850, Beijing, People's Republic of China aut Pang Wei Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut Zhan Rui Institute of Basic Medical Sciences, 100850, Beijing, People's Republic of China aut Zhang Zhiqing Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut Wang Liqun Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut Gao Xiujie Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut Qian Lingjia Institute of Basic Medical Sciences, 100850, Beijing, People's Republic of China aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 483-493 1355-8145 18:4<483 2013 18 12192 https://doi.org/10.1007/s12192-013-0404-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0404-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leng Xue Institute of Medical Equipment, 300161, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xinxing Institute of Basic Medical Sciences, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Pang Wei Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Zhan Rui Institute of Basic Medical Sciences, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhiqing Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Wang Liqun Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Gao Xiujie Institute of Health & Environmental Medicine, 300050, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Qian Lingjia Institute of Basic Medical Sciences, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 483-493 1355-8145 18:4<483 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer