naa a22 4500 510746217 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-012-0394-7 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0394-7 Identification, genomic organization, and oxidative stress response of a sigma class glutathione S-transferase gene (AccGSTS1) in the honey bee, Apis cerana cerana [Elektronische Daten] [Huiru Yan, Haihong Jia, Hongru Gao, Xingqi Guo, Baohua Xu] Glutathione S-transferases (GSTs) are members of a multifunctional antioxidant enzyme superfamily that play pivotal roles in both detoxification and protection against oxidative damage caused by reactive oxygen species. In this study, a complementary DNA (cDNA) encoding a sigma class GST was identified in the Chinese honey bee, Apis cerana cerana (AccGSTS1). AccGSTS1 was constitutively expressed in all tissues of adult worker bees, including the brain, fat body, epidermis, muscle, and midgut, with particularly robust transcription in the fat body. Relative messenger RNA expression levels of AccGSTS1 at different developmental stages varied, with the highest levels of expression observed in adults. The potential function of AccGSTS1 in cellular defenses against abiotic stresses (cold, heat, UV, H2O2, HgCl2, and insecticides) was investigated. AccGSTS1 was significantly upregulated in response to all of the treatment conditions examined, although the induction levels were varied. Recombinant AccGSTS1 protein showed characteristic glutathione-conjugating catalytic activity toward 1-chloro-2,4-dinitrobenzene. Functional assays revealed that AccGSTS1 could remove H2O2, thereby protecting DNA from oxidative damage. Escherichia coli overexpressing AccGSTS1 showed long-term resistance under conditions of oxidative stress. Together, these results suggest that AccGSTS1 is a crucial antioxidant enzyme involved in cellular antioxidant defenses and honey bee survival. Cell Stress Society International, 2012 Glutathione-S-transferase nationallicence Oxidative stress nationallicence Antioxidant defense nationallicence Apis cerana cerana nationallicence Yan Huiru College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Jia Haihong College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Gao Hongru College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Guo Xingqi College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Xu Baohua College of Animal Science and Technology, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 415-426 1355-8145 18:4<415 2013 18 12192 https://doi.org/10.1007/s12192-012-0394-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0394-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Huiru College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Jia Haihong College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Gao Hongru College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Guo Xingqi College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Xu Baohua College of Animal Science and Technology, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 415-426 1355-8145 18:4<415 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer