naa a22 4500 510746225 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-013-0406-2 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0406-2 A novel Omega-class glutathione S -transferase gene in Apis cerana cerana [Elektronische Daten] molecular characterisation of GSTO2 and its protective effects in oxidative stress [Yuanying Zhang, Huiru Yan, Wenjing Lu, Yuzhen Li, Xingqi Guo, Baohua Xu] Oxidative stress may be the most significant threat to the survival of living organisms. Glutathione S-transferases (GSTs) serve as the primary defences against xenobiotic and peroxidative-induced oxidative damage. In contrast to other well-defined GST classes, the Omega-class members are poorly understood, particularly in insects. Here, we isolated and characterised the GSTO2 gene from Apis cerana cerana (AccGSTO2). The predicted transcription factor binding sites in the AccGSTO2 promoter suggested possible functions in early development and antioxidant defence. Real-time quantitative PCR (qPCR) and western blot analyses indicated that AccGSTO2 was highly expressed in larvae and was predominantly localised to the brain tissue in adults. Moreover, AccGSTO2 transcription was induced by various abiotic stresses. The purified recombinant AccGSTO2 exhibited glutathione-dependent dehydroascorbate reductase and peroxidase activities. Furthermore, it could prevent DNA damage. In addition, Escherichia coli overexpressing AccGSTO2 displayed resistance to long-term oxidative stress exposure in disc diffusion assays. Taken together, these results suggest that AccGSTO2 plays a protective role in counteracting oxidative stress. Cell Stress Society International, 2013 Apis cerana cerana nationallicence GSTO2 nationallicence Gene expression pattern nationallicence Oxidative stress nationallicence Biochemical properties nationallicence Zhang Yuanying State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Yan Huiru State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Lu Wenjing State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Li Yuzhen State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Guo Xingqi State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Xu Baohua College of Animal Science and Technology, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 503-516 1355-8145 18:4<503 2013 18 12192 https://doi.org/10.1007/s12192-013-0406-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0406-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yuanying State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Yan Huiru State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Lu Wenjing State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Li Yuzhen State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Guo Xingqi State Key Laboratory of Crop Biology College of Life Sciences, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Xu Baohua College of Animal Science and Technology, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 503-516 1355-8145 18:4<503 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer