naa a22 4500 510746241 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12192-013-0405-3 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0405-3 ERK signaling is triggered by hepatitis C virus E2 protein through DC-SIGN [Elektronische Daten] [Lan-Juan Zhao, Wen Wang, Hao Ren, Zhong-Tian Qi] Dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is a binding receptor for hepatitis C virus (HCV). Binding of HCV envelope protein E2 to target cells is a prerequisite to DC-SIGN-mediated signaling. Using cell lines with stable or transient expression of DC-SIGN, we investigated effects of soluble HCV E2 protein on ERK pathway. MEK and ERK are activated by the E2 in NIH3T3 cells stably expressing DC-SIGN. Treatment of the cells with antibody to DC-SIGN results in inhibition of the E2 binding as well as the E2-induced MEK and ERK activation. In HEK293T cells transiently expressing DC-SIGN, activation of MEK and ERK is also induced by the E2. Activation of ERK pathway by HCV E2 through DC-SIGN provides useful information for understanding cellular receptor-mediated signaling. Cell Stress Society International, 2013 DC-SIGN nationallicence HCV nationallicence Envelope protein E2 nationallicence ERK nationallicence Zhao Lan-Juan Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut Wang Wen Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut Ren Hao Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut Qi Zhong-Tian Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 495-501 1355-8145 18:4<495 2013 18 12192 https://doi.org/10.1007/s12192-013-0405-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0405-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Lan-Juan Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wen Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Ren Hao Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Qi Zhong-Tian Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/4(2013-07-01), 495-501 1355-8145 18:4<495 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer