naa a22 4500 51074625X CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0418-y doi (NATIONALLICENCE)springer-10.1007/s12192-013-0418-y Isolated hearts treated with skeletal muscle homogenates exhibit altered function [Elektronische Daten] [Alex Di Battista, Marius Locke] Skeletal muscle fiber damage and necrosis can result in the release of intracellular molecules into the extracellular environment. These molecules, termed damage-associated molecular patterns (DAMPs), can act as signals capable of initiating immune and/or inflammatory responses through interactions with pattern recognition receptors. To investigate whether skeletal muscle DAMPs interact with the heart and alter cardiac function, isolated rat hearts were perfused for 75min with buffer containing 1μg/ml of either soleus (slow), white gastrocnemius (WG, fast), or heat-stressed white gastrocnemius (HSWG) skeletal muscle homogenates. Left ventricular developed pressure (LVDP) and rates of pressure increase/decrease (±dP/dt) were measured using the Langendorff technique. Compared to controls, no changes in LVDP or +dP/dt were observed over the 75-min perfusion when homogenates from the WG muscles were added. In contrast, at 30min and thereafter, a decreased LVDP and +dP/dt was observed in the hearts treated with soleus muscle homogenates. The hearts treated with HSWG homogenates also showed a decrease in LVDP from 45min until the end of perfusion. These results suggest that molecules present in slow muscle and heat-stressed muscle are capable of altering cardiac function. Thus, muscle fiber type and/or heat shock protein content of skeletal muscles may be factors that influence cardiac function following skeletal muscle damage. Cell Stress Society International, 2013 Di Battista Alex Faculty of Kinesiology and Physical Education, University of Toronto, 55 Harbord Street, M5S 2W6, Toronto, Ontario, Canada aut Locke Marius Faculty of Kinesiology and Physical Education, University of Toronto, 55 Harbord Street, M5S 2W6, Toronto, Ontario, Canada aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 675-681 1355-8145 18:5<675 2013 18 12192 https://doi.org/10.1007/s12192-013-0418-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0418-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Battista Alex Faculty of Kinesiology and Physical Education, University of Toronto, 55 Harbord Street, M5S 2W6, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Locke Marius Faculty of Kinesiology and Physical Education, University of Toronto, 55 Harbord Street, M5S 2W6, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 675-681 1355-8145 18:5<675 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer