naa a22 4500 510746284 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0415-1 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0415-1 Impact of hyperthermic intraperitoneal chemotherapy on Hsp27 protein expression in serum of patients with peritoneal carcinomatosis [Elektronische Daten] [Vahan Kepenekian, Marie-Thérèse Aloy, Nicolas Magné, Guillaume Passot, Emma Armandy, Evelyne Decullier, Annie Sayag-Beaujard, François-Noël Gilly, Olivier Glehen, Claire Rodriguez-Lafrasse] Despite the strong rationale for combining cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC) in patients with peritoneal carcinomatosis, thermotolerance and chemoresistance might result from heat shock protein overexpression. The aim of the present study was thus to determine whether the heat shock protein 27 (Hsp27), a potential factor in resistance to treatment, could have a higher level in serum from patients under this combined therapy. Patients receiving CRS plus HIPEC for peritoneal carcinomatosis (group 1), patients with cancer or a history of cancer undergoing abdominal surgery (group 2), and patients without malignancies undergoing abdominal surgery (group 3) were included. Hsp27 serum levels were determined before and at different times following CRS and HIPEC using enzyme-linked immunosorbent assay. In group 1 (n = 25), the high Hsp27 levels, observed at the end of surgery compared with before (p < 0.0001), decreased during HIPEC, but remained significantly higher than before surgery (p < 0.0005). In groups 2 (n = 11) and 3 (n = 15), surgery did not significantly increase Hsp27 levels. A targeted molecular strategy, inhibiting Hsp27 expression in tumor tissue, could significantly reduce resistance to the combined CRS plus HIPEC treatment. This approach should be further assessed in a clinical phase I trial. Cell Stress Society International, 2013 Colorectal cancer nationallicence Heat shock protein 27 (Hsp7) nationallicence Hyperthermic intraperitoneal chemotherapy (HIPEC) nationallicence Ovarian cancer nationallicence Peritoneal carcinomatosis nationallicence Kepenekian Vahan EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Aloy Marie-Thérèse EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Magné Nicolas EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Passot Guillaume EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Armandy Emma EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Decullier Evelyne Medical Information, Evaluation, Research Pole (IMER Pole), Hospices Civils de Lyon, Lyon, France aut Sayag-Beaujard Annie EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Gilly François-Noël EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Glehen Olivier EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Rodriguez-Lafrasse Claire EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 623-630 1355-8145 18:5<623 2013 18 12192 https://doi.org/10.1007/s12192-013-0415-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0415-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kepenekian Vahan EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Aloy Marie-Thérèse EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Magné Nicolas EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Passot Guillaume EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Armandy Emma EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Decullier Evelyne Medical Information, Evaluation, Research Pole (IMER Pole), Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Sayag-Beaujard Annie EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Gilly François-Noël EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Glehen Olivier EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 700 1- Rodriguez-Lafrasse Claire EMR3738, Faculté de Médecine Lyon-Sud, Université Lyon 1, BP12 69921, Oullins Cedex, France aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 623-630 1355-8145 18:5<623 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer