naa a22 4500 510746314 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0408-0 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0408-0 In MMTV-Her-2/neu transgenic mammary tumors the absence of caveolin-1−/− alters PTEN and NHERF1 but not β-catenin expression [Elektronische Daten] [F. Cuello-Carrión, Niubys Cayado-Gutiérrez, Anthony Natoli, Christina Restall, Robin Anderson, Silvina Nadin, Daiana Alvarez-Olmedo, Gisela Castro, Francisco Gago, Mariel Fanelli, Daniel Ciocca] In a recent study, we have shown that in mammary tumors from mice lacking the Cav-1 gene, there are alterations in specific heat shock proteins as well as in tumor development. With this in mind, we have now investigated other proteins in the same mammary mouse tumor model (Her-2/neu expressing mammary tumors from Cav-1 wild type and Cav-1 null mice), to further comprehend the complex tumor-stroma mechanisms involved in regulating stress responses during tumor development. In this tumor model the cancer cells always lacked of Cav-1, so the KO influenced the Cav-1 in the stroma. By immunohistochemistry, we have found a striking co-expression of β-catenin and Her-2/neu in the tumor cells. The absence of Cav-1 in the tumor stroma had no effect on expression or localization of β-catenin and Her-2/neu. Both proteins appeared co-localized at the cell surface during tumor development and progression. Since Her-2/neu activation induces MTA1, we next evaluated MTA1 in the mouse tumors. Although this protein was found in numerous nuclei, the absence of Cav-1 did not alter its expression level. In contrast, significantly more PTEN protein was noted in the tumors lacking Cav-1 in the stroma, with the protein localized mainly in the nuclei. P-Akt levels were relatively low in tumors from both Cav-1 WT and Cav-1 KO mice. There was also an increase in nuclear NHERF1 expression levels in the tumors arising from Cav-1 KO mice. The data obtained in the MMTV-neu model are consistent with a role for Cav-1 in adjacent breast cancer stromal cells in modulating the expression and localization of important proteins implicated in tumor cell behavior. Cell Stress Society International, 2013 Caveolin-1 nationallicence PTEN nationallicence NHERF1 nationallicence β-Catenin nationallicence Breast cancer nationallicence Her-2/neu nationallicence Oncogene nationallicence Cav-1 : Caveolin-1 nationallicence EMT : Epithelial to mesenchymal transition nationallicence ER : Estrogen receptor nationallicence Her-2/neu : c-erbB-2 nationallicence HSP : Heat shock protein nationallicence IHC : Immunohistochemistry nationallicence KO : Knockout nationallicence MMTV : Murine mammary tumor virus nationallicence MTA1 : Metastasis associated protein 1 nationallicence NHERF1 : Na+/H+ exchanger regulator factor 1 nationallicence PR : Progesterone receptor nationallicence PTEN : Phosphatase and tensin homologue deleted on chromosome 10 nationallicence WT : Wild type nationallicence Cuello-Carrión F. Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Cayado-Gutiérrez Niubys Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Natoli Anthony Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Victoria, Australia aut Restall Christina Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Victoria, Australia aut Anderson Robin Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Victoria, Australia aut Nadin Silvina Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Alvarez-Olmedo Daiana Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Castro Gisela Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Gago Francisco Medical School, National University of Cuyo, and Italian Hospital, Mendoza, Argentina aut Fanelli Mariel Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Ciocca Daniel Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 559-567 1355-8145 18:5<559 2013 18 12192 https://doi.org/10.1007/s12192-013-0408-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0408-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cuello-Carrión F. Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 700 1- Cayado-Gutiérrez Niubys Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 700 1- Natoli Anthony Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Victoria, Australia aut NATIONALLICENCE 700 1- Restall Christina Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Victoria, Australia aut NATIONALLICENCE 700 1- Anderson Robin Metastasis Research Laboratory, Peter MacCallum Cancer Centre, Victoria, Australia aut NATIONALLICENCE 700 1- Nadin Silvina Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 700 1- Alvarez-Olmedo Daiana Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 700 1- Castro Gisela Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 700 1- Gago Francisco Medical School, National University of Cuyo, and Italian Hospital, Mendoza, Argentina aut NATIONALLICENCE 700 1- Fanelli Mariel Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 700 1- Ciocca Daniel Laboratory of Oncology, Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT)-National Research Council of Argentina (CONICET), Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 559-567 1355-8145 18:5<559 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer