naa a22 4500 510746322 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0417-z doi (NATIONALLICENCE)springer-10.1007/s12192-013-0417-z Visfatin induces cholesterol accumulation in macrophages through up-regulation of scavenger receptor-A and CD36 [Elektronische Daten] [Fenghua Zhou, Yunyun Pan, Zhiyong Huang, Yuhua Jia, Xiaoshan Zhao, Yuyao Chen, Jianxin Diao, Qiang Wan, Xiaobing Cui] As a new potential inflammatory mediator, visfatin plays an important role in inflammation and atherosclerosis. The formation of macrophage-derived foam cells occurs at the early stage of atherosclerosis and underlies the visible fatty streak. Recent studies have indicated that visfatin may be associated with the development of foam cells, but its exact effect and molecular mechanism remain unknown. This study aims to study the effect of visfatin on foamy cell formation and its underlying molecular mechanism. Visfatin levels were determined in apolipoprotein E (ApoE) knockout (KO) mice on a western diet for 16weeks. Effects of visfatin in cholesterol accumulation were studied both in vivo and in vitro. The levels of scavenger receptors located in macrophage surface were measured in RAW264.7 cells after treatment with visfatin. Visfatin levels were much higher in ApoE KO mice than that in the control mice. Meanwhile, oxidized low-density lipoprotein induces both visfatin release from RAW264.7 cells and its cellular levels within 24h. Visfatin promotes lipid accumulation mainly through excessive cholesterol uptake not only in RAW264.7 cells but also in peritoneal macrophages isolated from ApoE KO mice. Furthermore, visfatin induces the activation of scavenger receptors (SR)-A and cluster of differentiation (CD)36, but not that of SR-BI, ATP-binding cassette transporter (ABC)A1, or ABCG1 in RAW264.7 cells. Both transcriptional and posttranscriptional regulation may work in concert to mediate the expression of SR-A and CD36 in visfatin-treated cells. Visfatin induces cholesterol accumulation in macrophages and accelerates the process of atherosclerosis mainly through modulating the expression of SR-A and CD36. Cell Stress Society International, 2013 Atherosclerosis nationallicence Visfatin nationallicence Foam cell nationallicence Scavenger receptor nationallicence Zhou Fenghua Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Pan Yunyun Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Huang Zhiyong People's Liberation Army Air Force Radar Academy, 288 Huangpu Street, 430019, Wuhan, Hubei, China aut Jia Yuhua Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Zhao Xiaoshan Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Chen Yuyao School of Traditional Chinese Medicine, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Diao Jianxin School of Traditional Chinese Medicine, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Wan Qiang Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Cui Xiaobing Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 643-652 1355-8145 18:5<643 2013 18 12192 https://doi.org/10.1007/s12192-013-0417-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0417-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Fenghua Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Pan Yunyun Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Huang Zhiyong People's Liberation Army Air Force Radar Academy, 288 Huangpu Street, 430019, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Jia Yuhua Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Zhao Xiaoshan Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Chen Yuyao School of Traditional Chinese Medicine, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Diao Jianxin School of Traditional Chinese Medicine, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Wan Qiang Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 700 1- Cui Xiaobing Department of Traditional Chinese Medicine, Nan fang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, 510515, Guangzhou, Guangdong, China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 643-652 1355-8145 18:5<643 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer