naa a22 4500 510746330 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0426-y doi (NATIONALLICENCE)springer-10.1007/s12192-013-0426-y Dynamic O -GlcNAcylation and its roles in the cellular stress response and homeostasis [Elektronische Daten] [Jennifer Groves, Albert Lee, Gokben Yildirir, Natasha Zachara] O-linked N-acetyl-β-d-glucosamine (O-GlcNAc) is a ubiquitous and dynamic post-translational modification known to modify over 3,000 nuclear, cytoplasmic, and mitochondrial eukaryotic proteins. Addition of O-GlcNAc to proteins is catalyzed by the O-GlcNAc transferase and is removed by a neutral-N-acetyl-β-glucosaminidase (O-GlcNAcase). O-GlcNAc is thought to regulate proteins in a manner analogous to protein phosphorylation, and the cycling of this carbohydrate modification regulates many cellular functions such as the cellular stress response. Diverse forms of cellular stress and tissue injury result in enhanced O-GlcNAc modification, or O-GlcNAcylation, of numerous intracellular proteins. Stress-induced O-GlcNAcylation appears to promote cell/tissue survival by regulating a multitude of biological processes including: the phosphoinositide 3-kinase/Akt pathway, heat shock protein expression, calcium homeostasis, levels of reactive oxygen species, ER stress, protein stability, mitochondrial dynamics, and inflammation. Here, we will discuss the regulation of these processes by O-GlcNAc and the impact of such regulation on survival in models of ischemia reperfusion injury and trauma hemorrhage. We will also discuss the misregulation of O-GlcNAc in diseases commonly associated with the stress response, namely Alzheimer's and Parkinson's diseases. Finally, we will highlight recent advancements in the tools and technologies used to study the O-GlcNAc modification. Cell Stress Society International, 2013 O -GlcNAc nationallicence Stress nationallicence Signal transduction nationallicence O -GlcNAc transferase nationallicence O -GlcNAcase nationallicence Post-translational modification nationallicence 4-OHT : 4-Hydroxytamoxifen nationallicence AD : Alzheimer's disease nationallicence APP : Amyloid β precursor protein nationallicence Aβ : Amyloid β nationallicence Bcl-2 : B-cell lymphoma 2 nationallicence BEMAD : Beta elimination and Michael addition nationallicence CaMK : Ca2+/calmodulin-dependent kinase nationallicence CCE : Capacitative Ca2+ entry nationallicence CID : Collision-induced dissociation nationallicence DON : 6-Diazo-5-oxo-L-norleucine nationallicence Emeg32 : Glucosamine-6-phosphate acetyltransferase nationallicence eNOS : Endothelial nitric oxide synthase nationallicence ESI : Electrospray ionization nationallicence ETD : Electron transfer dissociation nationallicence FDGlcNAc : Fluorescein di-N-acetyl-β-d-glucosaminide nationallicence Foxo : Forkhead box nationallicence FRET : Förster resonance energy transfer nationallicence GalNAz : N-azidoacetylgalactosamine nationallicence GalT1 : UDP-Gal/GlcNAcβ-1,4-galactosyltransferase nationallicence GE : Gel electrophoresis nationallicence GFAT : Glutamine/fructose-6-phosphate amidotransferase nationallicence GlcNAz : N-azidoacetylglucosamine nationallicence GSK3β : Glycogen synthase kinase 3β nationallicence HAT : Histone acetyltransferase nationallicence HBP : Hexosamine biosynthetic pathway nationallicence Hex : Hexosaminidase nationallicence HK : Hexokinase nationallicence HSC : Heat shock cognate nationallicence HSF : Heat shock factor nationallicence HSP : Heat shock protein nationallicence I/R : Ischemia-reperfusion nationallicence IKKβ : Inhibitor of NFκB kinase nationallicence IL : Interleukin nationallicence IP3 : Inositol (3,4,5)-trisphosphate nationallicence IRS-1 : Insulin receptor substrate 1 nationallicence K18 : Cytokeratin 18 nationallicence LC : Liquid chromatography nationallicence LWAC : Lectin weak affinity chromatography nationallicence MALDI : Matrix-assisted laser desorption ionization nationallicence MAP3K7 : Mitogen-activated protein kinase kinase kinase 7 nationallicence MAPK : Mitogen-activated protein kinase nationallicence Mgea5 : Meningioma-expressed antigen 5 nationallicence mOGT : Mitochondrial OGT nationallicence mPTP : Mitochondrial permeability transition pore nationallicence MS : Mass spectrometry nationallicence MS/MS : Tandem mass spectrometry nationallicence nCl : Negative chemical ionization nationallicence ncOGT : Nuclear/cytoplasmic OGT nationallicence NFκB : Nuclear factor κB nationallicence NO : Nitric oxide nationallicence O -GlcNAc : O-linked N-acetyl-β-d-glucosamine nationallicence O -GlcNAcase (OGA) : N-acetyl-β-glucosaminidase nationallicence O -GlcNAcylated : O-GlcNAc-modified nationallicence OGT ( O -GlcNAc transferase) : UDP-N-acetylglucosamine/peptide N-acetylglucosaminyl-transferase nationallicence PD : Parkinson's disease nationallicence PGC1α : Peroxisome proliferator-activated receptor gamma coactivator 1-α nationallicence Pgm3 : N-acetylglucosamine-phosphate mutase nationallicence PI3K : Phosphoinositide 3-kinase nationallicence PIP3 : Phosphatidylinositol (3,4,5)-trisphosphate nationallicence PNGase : F Peptide N-glycosidase F nationallicence p NP-β-GlcNAc : p-Nitrophenol-N-acetyl-β-d-glucosamine nationallicence PRMT4/Carm1 : Protein arginine methyltransferase 4 nationallicence PTM : Post-translational modification nationallicence PUGNAc : O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino-N-phenylcarbamate nationallicence QTOF : Quadrupole time-of-flight nationallicence Rb : Retinoblastoma protein nationallicence ROS : Reactive oxygen species nationallicence RT-PCR : Real-time polymerase chain reaction nationallicence SILAC : Stable isotope labeling with amino acids in cell culture nationallicence STZ : Streptozotocin nationallicence sWGA : Succinylated WGA nationallicence TAB1 : TAK1-binding protein nationallicence TAD : Transactivation domain nationallicence TAK1 : TGF-β-activated kinase I nationallicence TGF : Transforming growth factor nationallicence TMG : (thiamet-G) 2-Ethylamino-3aR, 6S, 7R, 7aR-tetrahydro-5R-hydroxymethyl-5H-pyrano[3, 2-d]thiazole-6,7-diol nationallicence TNF-α : Tumor necrosis factor-α nationallicence TRP : Tetratricopeptide nationallicence Uap1 : UDP-GlcNAc pyrophosphorylase nationallicence UDP : Uridine diphosphate nationallicence UL32 : Human cytomegalovirus tegument basic phosphoprotein nationallicence VDAC : Voltage-dependent anion channel nationallicence WGA : Wheat germ agglutinin nationallicence Groves Jennifer The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut Lee Albert The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut Yildirir Gokben The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut Zachara Natasha The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 535-558 1355-8145 18:5<535 2013 18 12192 https://doi.org/10.1007/s12192-013-0426-y text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0426-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Groves Jennifer The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Lee Albert The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Yildirir Gokben The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Zachara Natasha The Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, 21205-2185, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 535-558 1355-8145 18:5<535 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer