naa a22 4500 510746349 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0409-z doi (NATIONALLICENCE)springer-10.1007/s12192-013-0409-z Butyric acid retention in gingival tissue induces oxidative stress in jugular blood mitochondria [Elektronische Daten] [Marni Cueno, Kenichi Imai, Noriko Matsukawa, Takamitsu Tsukahara, Tomoko Kurita-Ochiai, Kuniyasu Ochiai] Butyric acid (BA) is a major extracellular metabolite produced by anaerobic periodontopathic bacteria and is commonly deposited in the gingival tissue. BA induces mitochondrial oxidative stress in vitro; however, its effects in vivo were never elucidated. Here, we determined the effects of butyric acid retention in the gingival tissues on oxidative stress induction in the jugular blood mitochondria. We established that BA injected in the rat gingival tissue has prolonged retention in gingival tissues. Blood taken at 0, 60, and 180min after BA injection was used for further analysis. We isolated blood mitochondria, verified its purity, and measured hydrogen peroxide (H2O2), heme, superoxide (SOD), and catalase (CAT) to determine BA effects. We found that H2O2, heme, SOD, and CAT levels all increased after BA injection. This would insinuate that mitochondrial oxidative stress was induced ascribable to BA. Cell Stress Society International, 2013 Butyric acid nationallicence Gingival tissue nationallicence Mitochondria nationallicence Rat blood nationallicence Reactive oxygen species homeostasis nationallicence Cueno Marni Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Imai Kenichi Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Matsukawa Noriko Kyoto Institute of Nutrition and Pathology Inc., Tachikawa Ujitawara, 610-0231, Kyoto, Japan aut Tsukahara Takamitsu Kyoto Institute of Nutrition and Pathology Inc., Tachikawa Ujitawara, 610-0231, Kyoto, Japan aut Kurita-Ochiai Tomoko Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan aut Ochiai Kuniyasu Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 661-665 1355-8145 18:5<661 2013 18 12192 https://doi.org/10.1007/s12192-013-0409-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0409-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cueno Marni Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Imai Kenichi Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsukawa Noriko Kyoto Institute of Nutrition and Pathology Inc., Tachikawa Ujitawara, 610-0231, Kyoto, Japan aut NATIONALLICENCE 700 1- Tsukahara Takamitsu Kyoto Institute of Nutrition and Pathology Inc., Tachikawa Ujitawara, 610-0231, Kyoto, Japan aut NATIONALLICENCE 700 1- Kurita-Ochiai Tomoko Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Ochiai Kuniyasu Department of Microbiology, Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 661-665 1355-8145 18:5<661 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer