naa a22 4500 510746365 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12192-013-0407-1 doi (NATIONALLICENCE)springer-10.1007/s12192-013-0407-1 Human Hsp40 proteins, DNAJA1 and DNAJA2, as potential targets of the immune response triggered by bacterial DnaJ in rheumatoid arthritis [Elektronische Daten] [Agnieszka Kotlarz, Stefan Tukaj, Konrad Krzewski, Elzbieta Brycka, Barbara Lipinska] Hsp40 proteins of bacterial and human origin are suspected to be involved in the pathogenesis of rheumatoid arthritis (RA). It has been shown that sera of RA patients contain increased levels of antibodies directed to bacterial and human Hsp40s. The aim of this work was to explore immunological similarities between the bacterial (DnaJ) and human (DNAJA1 and DNAJA2) Hsp40 proteins in relation to their possible involvement in the RA. Using polyclonal antibodies directed against a full-length DnaJ or its domains, against DNAJA1 and DNAJA2, as well as monoclonal anti-DnaJ antibodies, we found immunological similarities between the bacterial and human Hsp40s. Both ELISA and Western blotting showed that these similarities were not restricted to the conserved J domains but were also present in the C-terminal variable regions. We also found a positive correlation between the levels of the anti-DnaJ and anti-DNAJA1 antibodies in the sera of RA patients. This finding supports the molecular mimicry hypothesis that human Hsp40 could be the targets of antibodies originally directed against bacterial DnaJ in RA. The Author(s), 2013 Anti-Hsp40 autoantibodies nationallicence Hsp40 in rheumatoid arthritis nationallicence Cross-reactivity of anti-Hsp40 antibodies nationallicence Anti-DNAJ monoclonal antibodies nationallicence Molecular mimicry nationallicence Kotlarz Agnieszka Department of Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland aut Tukaj Stefan Department of Plant Physiology and Biotechnology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland aut Krzewski Konrad Department of Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland aut Brycka Elzbieta Department of Molecular Virology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland aut Lipinska Barbara Department of Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland aut Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 653-659 1355-8145 18:5<653 2013 18 12192 https://doi.org/10.1007/s12192-013-0407-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-013-0407-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kotlarz Agnieszka Department of Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland aut NATIONALLICENCE 700 1- Tukaj Stefan Department of Plant Physiology and Biotechnology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland aut NATIONALLICENCE 700 1- Krzewski Konrad Department of Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland aut NATIONALLICENCE 700 1- Brycka Elzbieta Department of Molecular Virology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland aut NATIONALLICENCE 700 1- Lipinska Barbara Department of Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/5(2013-09-01), 653-659 1355-8145 18:5<653 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer