naa a22 4500 510746411 CHVBK 20180411083047.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12192-012-0370-2 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0370-2 Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects [Elektronische Daten] [Kedong Song, Hai Wang, Bowen Zhang, Mayasari Lim, YingChao Liu, Tianqing Liu] In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects. Cell Stress Society International, 2012 Rotating wall vessel bioreactor nationallicence FLUENT software nationallicence Stress stimulation nationallicence Tissue-engineered bones nationallicence Animal experiment nationallicence Song Kedong Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut Wang Hai Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut Zhang Bowen Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut Lim Mayasari Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore aut Liu YingChao Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut Liu Tianqing Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 193-201 1355-8145 18:2<193 2013 18 12192 https://doi.org/10.1007/s12192-012-0370-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0370-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Kedong Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- Wang Hai Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- Zhang Bowen Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- Lim Mayasari Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Liu YingChao Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- Liu Tianqing Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 193-201 1355-8145 18:2<193 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer