naa a22 4500 510746470 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12192-012-0396-5 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0396-5 TAC from Mycobacterium tuberculosis [Elektronische Daten] a paradigm for stress-responsive toxin-antitoxin systems controlled by SecB-like chaperones [Ambre Sala, Virginie Calderon, Patricia Bordes, Pierre Genevaux] Bacterial type II toxin-antitoxins (TAs) are two-component systems that modulate growth in response to specific stress conditions, thus promoting adaptation and persistence. The major human pathogen Mycobacterium tuberculosis potentially encodes 75 TAs and it has been proposed that persistence induced by active toxins might be relevant for its pathogenesis. In this work, we focus on the newly discovered toxin-antitoxin-chaperone (TAC) system of M. tuberculosis, an atypical stress-responsive TA system tightly controlled by a molecular chaperone that shows similarity to the canonical SecB chaperone involved in Sec-dependent protein export in Gram-negative bacteria. We performed a large-scale genome screening to reconstruct the evolutionary history of TAC systems and found that TAC is not restricted to mycobacteria and seems to have disseminated in diverse taxonomic groups by horizontal gene transfer. Our results suggest that TAC chaperones are evolutionary related to the solitary chaperone SecB and have diverged to become specialized toward their cognate antitoxins. The Author(s), 2012 SecB chaperones nationallicence Sec translocon nationallicence Toxin-antitoxin systems nationallicence Stress response nationallicence HigBA nationallicence MqsRA nationallicence HicAB nationallicence TA : Toxin-antitoxin nationallicence TAC : Toxin-antitoxin-chaperone nationallicence AC : Antitoxin-chaperone nationallicence IPTG : Isopropyl β-d-1-thiogalactopyranoside nationallicence MTBC : Mycobacterium tuberculosis complex nationallicence MCL : Markov clustering nationallicence HTH : Helix-turn-helix nationallicence Sala Ambre Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut Calderon Virginie Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut Bordes Patricia Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut Genevaux Pierre Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 129-135 1355-8145 18:2<129 2013 18 12192 https://doi.org/10.1007/s12192-012-0396-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0396-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sala Ambre Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut NATIONALLICENCE 700 1- Calderon Virginie Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut NATIONALLICENCE 700 1- Bordes Patricia Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut NATIONALLICENCE 700 1- Genevaux Pierre Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique and Université Paul Sabatier, 31000, Toulouse, France aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 129-135 1355-8145 18:2<129 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer